Radioiodinated (aminostyryl)pyridinium (ASP) dyes: New cell membrane probes for labeling mixed leukocytes and lymphocytes for diagnostic imaging

Carol Lambert, Ronnie C. Mease, Lee Avren, Truc Le, Hassan Sabet, John G. McAfee

Research output: Contribution to journalArticle

Abstract

We prepared [125I/131I]iodo-(aminostyryl)pyridinium dyes from tributylstannyl precursors. ASP 7a and 7b labeled leukocytes ex vivo (70-94%) using saline with or without washing plasma from cells, Viability of peripheral blood lymphocytes (PBLs) (dogs, rats) and splenic lymphocytes (rats) labeled with 7a and 7b (71-82%) was unchanged after labeling (≤88%). Canine 7b-leukocytes showed higher uptake in inflammatory lesions than did 111In oxine leukocytes. At 3 h, aspirates contained more radioiodine than 111In (1.65:1 to 22:1) and radioiodine was cell bound. ROI measurements (3 h) gave abscess to contralateral knee ratios of 12.3 and 10.6 for 131I-7b vs. 4.8 and 2.3 for 111In-oxine.

Original languageEnglish (US)
Pages (from-to)417-427
Number of pages11
JournalNuclear Medicine and Biology
Volume23
Issue number4
DOIs
StatePublished - May 1996
Externally publishedYes

Keywords

  • (Aminostyryl)pyridinium dyes
  • Inflammation imaging
  • Leukocyte labeling
  • Lymphocyte labeling
  • Radioiodinated cell membrane probes

ASJC Scopus subject areas

  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

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