Rac1 is required for cell proliferation and G2/M progression

Kimberly A. Moore; Rachna Sethi; A. Masharn Doanes; Thomas M. Johnson; John B. Pracyk; Martha Kirby; Kaikobad Irani; Pascal J. Goldschmidt-Clermont; Toren Finkel

Rac1 is required for cell proliferation and G2/M progression

Kimberly A. Moore, Rachna Sethi, A. Masharn Doanes, Thomas M. Johnson, John B. Pracyk, Martha Kirby, Kaikobad Irani, Pascal J. Goldschmidt-Clermont, Toren Finkel

Research output: Contribution to journal › Article › peer-review

68 Scopus citations

Abstract

We have transiently expressed a dominant negative form of rac1 (N17rac1) using adenoviral-mediated gene transfer. The level of N17rac1 expression is demonstrated to be proportional to the multiplicity of infection. Expression of N17rac1 in Rat 2 fibroblasts results in cytostatic growth arrest. Cell-cycle analysis demonstrates that cells expressing N17rac1 accumulate in G2/M. These results suggest that rac1 is required for cell proliferation and provide the first demonstration in mammalian cells of a role for small GTP-binding proteins in the G2/M transition.

Original language	English (US)
Pages (from-to)	17-20
Number of pages	4
Journal	Biochemical Journal
Volume	326
Issue number	1
State	Published - Aug 15 1997
Externally published	Yes

ASJC Scopus subject areas

Biochemistry

Cite this

@article{0a2d13a91c844e859a0c5a644e9f302b,

title = "Rac1 is required for cell proliferation and G2/M progression",

abstract = "We have transiently expressed a dominant negative form of rac1 (N17rac1) using adenoviral-mediated gene transfer. The level of N17rac1 expression is demonstrated to be proportional to the multiplicity of infection. Expression of N17rac1 in Rat 2 fibroblasts results in cytostatic growth arrest. Cell-cycle analysis demonstrates that cells expressing N17rac1 accumulate in G2/M. These results suggest that rac1 is required for cell proliferation and provide the first demonstration in mammalian cells of a role for small GTP-binding proteins in the G2/M transition.",

author = "Moore, {Kimberly A.} and Rachna Sethi and Doanes, {A. Masharn} and Johnson, {Thomas M.} and Pracyk, {John B.} and Martha Kirby and Kaikobad Irani and Goldschmidt-Clermont, {Pascal J.} and Toren Finkel",

year = "1997",

month = aug,

day = "15",

language = "English (US)",

volume = "326",

pages = "17--20",

journal = "Biochemical Journal",

issn = "0264-6021",

publisher = "Portland Press Ltd.",

number = "1",

}

TY - JOUR

T1 - Rac1 is required for cell proliferation and G2/M progression

AU - Moore, Kimberly A.

AU - Sethi, Rachna

AU - Doanes, A. Masharn

AU - Johnson, Thomas M.

AU - Pracyk, John B.

AU - Kirby, Martha

AU - Irani, Kaikobad

AU - Goldschmidt-Clermont, Pascal J.

AU - Finkel, Toren

PY - 1997/8/15

Y1 - 1997/8/15

N2 - We have transiently expressed a dominant negative form of rac1 (N17rac1) using adenoviral-mediated gene transfer. The level of N17rac1 expression is demonstrated to be proportional to the multiplicity of infection. Expression of N17rac1 in Rat 2 fibroblasts results in cytostatic growth arrest. Cell-cycle analysis demonstrates that cells expressing N17rac1 accumulate in G2/M. These results suggest that rac1 is required for cell proliferation and provide the first demonstration in mammalian cells of a role for small GTP-binding proteins in the G2/M transition.

AB - We have transiently expressed a dominant negative form of rac1 (N17rac1) using adenoviral-mediated gene transfer. The level of N17rac1 expression is demonstrated to be proportional to the multiplicity of infection. Expression of N17rac1 in Rat 2 fibroblasts results in cytostatic growth arrest. Cell-cycle analysis demonstrates that cells expressing N17rac1 accumulate in G2/M. These results suggest that rac1 is required for cell proliferation and provide the first demonstration in mammalian cells of a role for small GTP-binding proteins in the G2/M transition.

UR - http://www.scopus.com/inward/record.url?scp=0030883583&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030883583&partnerID=8YFLogxK

M3 - Article

C2 - 9337845

AN - SCOPUS:0030883583

SN - 0264-6021

VL - 326

SP - 17

EP - 20

JO - Biochemical Journal

JF - Biochemical Journal

IS - 1

ER -

Rac1 is required for cell proliferation and G2/M progression

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this