R-spondin1 synergizes with Wnt3A in inducing osteoblast differentiation and osteoprotegerin expression

Wenyan Lu, Kyung Ah Kim, Jianzhong Liu, Arie Abo, Xu Feng, Xu Cao, Yonghe Li

Research output: Contribution to journalArticlepeer-review

Abstract

R-spondins are a new group of Wnt/β-catenin signaling agonists, however, the role of these proteins in bone remains unclear. We reported herein that R-sponin1 (Rspo1) acted synergistically with Wnt3A to activate Wnt/β-catenin signaling in the uncommitted mesenchymal C2C12 cells. Furthermore, we found that Rspo1 at concentrations as low as 10 ng/ml synergized strongly with Wnt3A to induce C2C12 osteoblastic differentiation and osteoprotegerin expression. These events were blocked by Wnt/β-catenin signaling antagonist Dickkopf-1. Finally, we demonstrated that Rspo1 synergized with Wnt3A to induce primary mouse osteoblast differentiation. Together, these findings suggest that Rpos1 may play an important role in bone remodeling.

Original languageEnglish (US)
Pages (from-to)643-650
Number of pages8
JournalFEBS Letters
Volume582
Issue number5
DOIs
StatePublished - Mar 5 2008

Keywords

  • Osteoblast differentiation
  • Osteoprotegerin
  • R-spondin1
  • Wnt signaling
  • Wnt3A

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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