Abstract
R-spondins are a new group of Wnt/β-catenin signaling agonists, however, the role of these proteins in bone remains unclear. We reported herein that R-sponin1 (Rspo1) acted synergistically with Wnt3A to activate Wnt/β-catenin signaling in the uncommitted mesenchymal C2C12 cells. Furthermore, we found that Rspo1 at concentrations as low as 10 ng/ml synergized strongly with Wnt3A to induce C2C12 osteoblastic differentiation and osteoprotegerin expression. These events were blocked by Wnt/β-catenin signaling antagonist Dickkopf-1. Finally, we demonstrated that Rspo1 synergized with Wnt3A to induce primary mouse osteoblast differentiation. Together, these findings suggest that Rpos1 may play an important role in bone remodeling.
Original language | English (US) |
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Pages (from-to) | 643-650 |
Number of pages | 8 |
Journal | FEBS Letters |
Volume | 582 |
Issue number | 5 |
DOIs | |
State | Published - Mar 5 2008 |
Externally published | Yes |
Keywords
- Osteoblast differentiation
- Osteoprotegerin
- R-spondin1
- Wnt signaling
- Wnt3A
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology