Quinone induced stimulation of hexose monophosphate shunt activity in the guinea pig lens

role of zeta-crystallin

Padmini Rao, J. Samuel Zigler

Research output: Contribution to journalArticle

Abstract

The response of the hexose monophosphate shunt (HMS) in organ-cultured guinea pig lens to 1,2-naphthoquinone and 5-hydroxy-1,4-naphthoquinone (juglone)_has been investigated. Both these compounds, which are substrates of guinea pig lens zeta-crystallin (NADPH:quinone oxidoreductase), were found to cause increases in the rate of 14CO2 production from 1-14C-labelled glucose. Exposure of lenses to 15 μM 1,2-naphthoquinone or 20 μM juglone yielded 5.9- and 7-fold stimulation of HMS activity, respectively. Unlike hydrogen peroxide-induced sitmulation of HMS activity, these effects were not abolished by preincubation with the glutathione reductase inhibitor, 1,3-bis(2-chloroethyl)-1 nitrosourea (BCNU). While hydrogen peroxide produced substantial decrements in lens glutathione (GSH) levels, incubation with quinones was not associated with a similar reduction in GSH concentration. Protein-bound NADPH content in quinone-exposed guinea pig lenses was decreased, with a concomitant increase in the amounts of free NADP+. This finding supported the involvement of zeta-crystallin bound NADPH in the in vivo enzymic reduction of quinones. Hydrogen peroxide, on the other hand, caused decreases in the level of free NADPH alone, serving to confirm our earlier inference that quinone stimulated increases in the guinea pig lens HMS could be mediated through zeta-crystallin NADPH:quinone oxidoreductase activity.

Original languageEnglish (US)
Pages (from-to)75-81
Number of pages7
JournalBiochimica et Biophysica Acta - General Subjects
Volume1116
Issue number1
DOIs
StatePublished - Mar 5 1992
Externally publishedYes

Fingerprint

zeta-Crystallins
Pentose Phosphate Pathway
Hexoses
NADP
Lenses
Guinea Pigs
Hydrogen Peroxide
Carmustine
Quinones
Oxidoreductases
Glutathione Reductase
Glutathione
benzoquinone
Glucose

Keywords

  • Hexose monophosphate shunt
  • Lens
  • NADP/NADPH
  • Quinone oxidoreductase
  • zeta-Crystallin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Quinone induced stimulation of hexose monophosphate shunt activity in the guinea pig lens : role of zeta-crystallin. / Rao, Padmini; Samuel Zigler, J.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 1116, No. 1, 05.03.1992, p. 75-81.

Research output: Contribution to journalArticle

@article{28417139f61c4cc28b7585ff765f128b,
title = "Quinone induced stimulation of hexose monophosphate shunt activity in the guinea pig lens: role of zeta-crystallin",
abstract = "The response of the hexose monophosphate shunt (HMS) in organ-cultured guinea pig lens to 1,2-naphthoquinone and 5-hydroxy-1,4-naphthoquinone (juglone)_has been investigated. Both these compounds, which are substrates of guinea pig lens zeta-crystallin (NADPH:quinone oxidoreductase), were found to cause increases in the rate of 14CO2 production from 1-14C-labelled glucose. Exposure of lenses to 15 μM 1,2-naphthoquinone or 20 μM juglone yielded 5.9- and 7-fold stimulation of HMS activity, respectively. Unlike hydrogen peroxide-induced sitmulation of HMS activity, these effects were not abolished by preincubation with the glutathione reductase inhibitor, 1,3-bis(2-chloroethyl)-1 nitrosourea (BCNU). While hydrogen peroxide produced substantial decrements in lens glutathione (GSH) levels, incubation with quinones was not associated with a similar reduction in GSH concentration. Protein-bound NADPH content in quinone-exposed guinea pig lenses was decreased, with a concomitant increase in the amounts of free NADP+. This finding supported the involvement of zeta-crystallin bound NADPH in the in vivo enzymic reduction of quinones. Hydrogen peroxide, on the other hand, caused decreases in the level of free NADPH alone, serving to confirm our earlier inference that quinone stimulated increases in the guinea pig lens HMS could be mediated through zeta-crystallin NADPH:quinone oxidoreductase activity.",
keywords = "Hexose monophosphate shunt, Lens, NADP/NADPH, Quinone oxidoreductase, zeta-Crystallin",
author = "Padmini Rao and {Samuel Zigler}, J.",
year = "1992",
month = "3",
day = "5",
doi = "10.1016/0304-4165(92)90131-D",
language = "English (US)",
volume = "1116",
pages = "75--81",
journal = "Biochimica et Biophysica Acta - General Subjects",
issn = "0304-4165",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Quinone induced stimulation of hexose monophosphate shunt activity in the guinea pig lens

T2 - role of zeta-crystallin

AU - Rao, Padmini

AU - Samuel Zigler, J.

PY - 1992/3/5

Y1 - 1992/3/5

N2 - The response of the hexose monophosphate shunt (HMS) in organ-cultured guinea pig lens to 1,2-naphthoquinone and 5-hydroxy-1,4-naphthoquinone (juglone)_has been investigated. Both these compounds, which are substrates of guinea pig lens zeta-crystallin (NADPH:quinone oxidoreductase), were found to cause increases in the rate of 14CO2 production from 1-14C-labelled glucose. Exposure of lenses to 15 μM 1,2-naphthoquinone or 20 μM juglone yielded 5.9- and 7-fold stimulation of HMS activity, respectively. Unlike hydrogen peroxide-induced sitmulation of HMS activity, these effects were not abolished by preincubation with the glutathione reductase inhibitor, 1,3-bis(2-chloroethyl)-1 nitrosourea (BCNU). While hydrogen peroxide produced substantial decrements in lens glutathione (GSH) levels, incubation with quinones was not associated with a similar reduction in GSH concentration. Protein-bound NADPH content in quinone-exposed guinea pig lenses was decreased, with a concomitant increase in the amounts of free NADP+. This finding supported the involvement of zeta-crystallin bound NADPH in the in vivo enzymic reduction of quinones. Hydrogen peroxide, on the other hand, caused decreases in the level of free NADPH alone, serving to confirm our earlier inference that quinone stimulated increases in the guinea pig lens HMS could be mediated through zeta-crystallin NADPH:quinone oxidoreductase activity.

AB - The response of the hexose monophosphate shunt (HMS) in organ-cultured guinea pig lens to 1,2-naphthoquinone and 5-hydroxy-1,4-naphthoquinone (juglone)_has been investigated. Both these compounds, which are substrates of guinea pig lens zeta-crystallin (NADPH:quinone oxidoreductase), were found to cause increases in the rate of 14CO2 production from 1-14C-labelled glucose. Exposure of lenses to 15 μM 1,2-naphthoquinone or 20 μM juglone yielded 5.9- and 7-fold stimulation of HMS activity, respectively. Unlike hydrogen peroxide-induced sitmulation of HMS activity, these effects were not abolished by preincubation with the glutathione reductase inhibitor, 1,3-bis(2-chloroethyl)-1 nitrosourea (BCNU). While hydrogen peroxide produced substantial decrements in lens glutathione (GSH) levels, incubation with quinones was not associated with a similar reduction in GSH concentration. Protein-bound NADPH content in quinone-exposed guinea pig lenses was decreased, with a concomitant increase in the amounts of free NADP+. This finding supported the involvement of zeta-crystallin bound NADPH in the in vivo enzymic reduction of quinones. Hydrogen peroxide, on the other hand, caused decreases in the level of free NADPH alone, serving to confirm our earlier inference that quinone stimulated increases in the guinea pig lens HMS could be mediated through zeta-crystallin NADPH:quinone oxidoreductase activity.

KW - Hexose monophosphate shunt

KW - Lens

KW - NADP/NADPH

KW - Quinone oxidoreductase

KW - zeta-Crystallin

UR - http://www.scopus.com/inward/record.url?scp=0026576432&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026576432&partnerID=8YFLogxK

U2 - 10.1016/0304-4165(92)90131-D

DO - 10.1016/0304-4165(92)90131-D

M3 - Article

VL - 1116

SP - 75

EP - 81

JO - Biochimica et Biophysica Acta - General Subjects

JF - Biochimica et Biophysica Acta - General Subjects

SN - 0304-4165

IS - 1

ER -