Quaternary structure of the mitochondrial TIM23 complex reveals dynamic association between Tim23p and other subunits

Nathan N. Alder, Jennifer Sutherland, Ashley I. Buhring, Robert E. Jensen, Arthur E. Johnson

Research output: Contribution to journalArticle

Abstract

Tim23p is an essential channel-forming component of the multisubunit TIM23 complex of the mitochondrial inner membrane that mediates protein import. Radiolabeled Tim23p monocysteine mutants were imported in vitro, incorporated into functional TIM23 complexes, and subjected to chemical cross-linking. Three regions of proximity between Tim23p and other subunits of the TIM23 complex were identified: Tim17p and the first transmembrane segment of Tim23p; Tim50p and the C-terminal end of the Tim23p hydrophilic region; and the entire hydrophilic domains of Tim23p molecules. These regions of proximity reversibly change in response to changes in membrane potential across the inner membrane and also when a translocating substrate is trapped in the TIM23 complex. These structural changes reveal that the macromolecular arrangement within the TIM23 complex is dynamic and varies with the physiological state of the mitochondrion.

Original languageEnglish (US)
Pages (from-to)159-170
Number of pages12
JournalMolecular Biology of the Cell
Volume19
Issue number1
DOIs
StatePublished - Jan 2008

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Membrane Potentials
Mitochondria
Membrane Proteins
Membranes
In Vitro Techniques

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Quaternary structure of the mitochondrial TIM23 complex reveals dynamic association between Tim23p and other subunits. / Alder, Nathan N.; Sutherland, Jennifer; Buhring, Ashley I.; Jensen, Robert E.; Johnson, Arthur E.

In: Molecular Biology of the Cell, Vol. 19, No. 1, 01.2008, p. 159-170.

Research output: Contribution to journalArticle

Alder, Nathan N. ; Sutherland, Jennifer ; Buhring, Ashley I. ; Jensen, Robert E. ; Johnson, Arthur E. / Quaternary structure of the mitochondrial TIM23 complex reveals dynamic association between Tim23p and other subunits. In: Molecular Biology of the Cell. 2008 ; Vol. 19, No. 1. pp. 159-170.
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