Quantum rod bioconjugates as targeted probes for confocal and two-photon fluorescence imaging of cancer cells

Ken Tye Yong, Jun Qian, Indrajit Roy, Hoon Hi Lee, Earl J. Bergey, Kenneth M. Tramposch, Sailing He, Mark T. Swihart, Anirban Maitra, Paras N. Prasad

Research output: Contribution to journalArticlepeer-review

Abstract

Live cell imaging using CdSe/CdS/ZnS quantum rods (QRs) as targeted optical probes is reported. The QRs, synthesized in organic media using a binary surfactant mixture, were dispersed in aqueous media using mercaptoundecanoic acid (MUA) and lysine. Transferrin (Tf) was linked to the QRs to produce QR-Tf bioconjugates that were used for targeted in vitro delivery to a human cancer cell line. Confocal and two-photon imaging were used to confirm receptor-mediated uptake of QR-Tf conjugates into the HeLa cells, which overexpress the transferrin receptor (TfR). Uptake was not observed with QRs that lacked Tf functionalization or with cells that were presaturated with free Tf and then treated with Tf-functionalized QRs.

Original languageEnglish (US)
Pages (from-to)761-765
Number of pages5
JournalNano Letters
Volume7
Issue number3
DOIs
StatePublished - Mar 2007

ASJC Scopus subject areas

  • Materials Science(all)
  • Electronic, Optical and Magnetic Materials
  • Chemistry (miscellaneous)

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