Quantum rod bioconjugates as targeted probes for confocal and two-photon fluorescence imaging of cancer cells

Ken Tye Yong; Jun Qian; Indrajit Roy; Hoon Hi Lee; Earl J. Bergey; Kenneth M. Tramposch; Sailing He; Mark T. Swihart; Anirban Maitra; Paras N. Prasad

doi:10.1021/nl063031m

Quantum rod bioconjugates as targeted probes for confocal and two-photon fluorescence imaging of cancer cells

Ken Tye Yong, Jun Qian, Indrajit Roy, Hoon Hi Lee, Earl J. Bergey, Kenneth M. Tramposch, Sailing He, Mark T. Swihart, Anirban Maitra, Paras N. Prasad

School of Medicine

Research output: Contribution to journal › Article › peer-review

177 Scopus citations

Abstract

Live cell imaging using CdSe/CdS/ZnS quantum rods (QRs) as targeted optical probes is reported. The QRs, synthesized in organic media using a binary surfactant mixture, were dispersed in aqueous media using mercaptoundecanoic acid (MUA) and lysine. Transferrin (Tf) was linked to the QRs to produce QR-Tf bioconjugates that were used for targeted in vitro delivery to a human cancer cell line. Confocal and two-photon imaging were used to confirm receptor-mediated uptake of QR-Tf conjugates into the HeLa cells, which overexpress the transferrin receptor (TfR). Uptake was not observed with QRs that lacked Tf functionalization or with cells that were presaturated with free Tf and then treated with Tf-functionalized QRs.

Original language	English (US)
Pages (from-to)	761-765
Number of pages	5
Journal	Nano Letters
Volume	7
Issue number	3
DOIs	https://doi.org/10.1021/nl063031m
State	Published - Mar 2007

ASJC Scopus subject areas

General Materials Science
Electronic, Optical and Magnetic Materials
Chemistry (miscellaneous)

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10.1021/nl063031m

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title = "Quantum rod bioconjugates as targeted probes for confocal and two-photon fluorescence imaging of cancer cells",

abstract = "Live cell imaging using CdSe/CdS/ZnS quantum rods (QRs) as targeted optical probes is reported. The QRs, synthesized in organic media using a binary surfactant mixture, were dispersed in aqueous media using mercaptoundecanoic acid (MUA) and lysine. Transferrin (Tf) was linked to the QRs to produce QR-Tf bioconjugates that were used for targeted in vitro delivery to a human cancer cell line. Confocal and two-photon imaging were used to confirm receptor-mediated uptake of QR-Tf conjugates into the HeLa cells, which overexpress the transferrin receptor (TfR). Uptake was not observed with QRs that lacked Tf functionalization or with cells that were presaturated with free Tf and then treated with Tf-functionalized QRs.",

author = "Yong, {Ken Tye} and Jun Qian and Indrajit Roy and Lee, {Hoon Hi} and Bergey, {Earl J.} and Tramposch, {Kenneth M.} and Sailing He and Swihart, {Mark T.} and Anirban Maitra and Prasad, {Paras N.}",

year = "2007",

month = mar,

doi = "10.1021/nl063031m",

language = "English (US)",

volume = "7",

pages = "761--765",

journal = "Nano Letters",

issn = "1530-6984",

publisher = "American Chemical Society",

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TY - JOUR

T1 - Quantum rod bioconjugates as targeted probes for confocal and two-photon fluorescence imaging of cancer cells

AU - Yong, Ken Tye

AU - Qian, Jun

AU - Roy, Indrajit

AU - Lee, Hoon Hi

AU - Bergey, Earl J.

AU - Tramposch, Kenneth M.

AU - He, Sailing

AU - Swihart, Mark T.

AU - Maitra, Anirban

AU - Prasad, Paras N.

PY - 2007/3

Y1 - 2007/3

N2 - Live cell imaging using CdSe/CdS/ZnS quantum rods (QRs) as targeted optical probes is reported. The QRs, synthesized in organic media using a binary surfactant mixture, were dispersed in aqueous media using mercaptoundecanoic acid (MUA) and lysine. Transferrin (Tf) was linked to the QRs to produce QR-Tf bioconjugates that were used for targeted in vitro delivery to a human cancer cell line. Confocal and two-photon imaging were used to confirm receptor-mediated uptake of QR-Tf conjugates into the HeLa cells, which overexpress the transferrin receptor (TfR). Uptake was not observed with QRs that lacked Tf functionalization or with cells that were presaturated with free Tf and then treated with Tf-functionalized QRs.

AB - Live cell imaging using CdSe/CdS/ZnS quantum rods (QRs) as targeted optical probes is reported. The QRs, synthesized in organic media using a binary surfactant mixture, were dispersed in aqueous media using mercaptoundecanoic acid (MUA) and lysine. Transferrin (Tf) was linked to the QRs to produce QR-Tf bioconjugates that were used for targeted in vitro delivery to a human cancer cell line. Confocal and two-photon imaging were used to confirm receptor-mediated uptake of QR-Tf conjugates into the HeLa cells, which overexpress the transferrin receptor (TfR). Uptake was not observed with QRs that lacked Tf functionalization or with cells that were presaturated with free Tf and then treated with Tf-functionalized QRs.

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JO - Nano Letters

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Quantum rod bioconjugates as targeted probes for confocal and two-photon fluorescence imaging of cancer cells

Abstract

ASJC Scopus subject areas

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