Quantum dot fluorescence characterizes the nanoscale organization of T cell receptors for antigen

Sarah Boyle, David L. Kolin, Joan Glick Bieler, Jonathan P Schneck, Paul W. Wiseman, Michael A Edidin

Research output: Contribution to journalArticle

Abstract

Changes in the clustering of surface receptors modulate cell responses to ligands. Hence, global measures of receptor clustering can be useful for characterizing cell states. Using T cell receptor for antigen as an example, we show that k-space image correlation spectroscopy of quantum dots blinking detects T cell receptor clusters on a scale of tens of nanometers and reports changes in clustering after T cell activation. Our results offer a general approach to the global analysis of lateral organization and receptor clustering in single cells, and can thus be applied when the cell type of interest is rare.

Original languageEnglish (US)
JournalBiophysical Journal
Volume101
Issue number11
DOIs
StatePublished - Dec 7 2011

Fingerprint

Quantum Dots
T-Cell Antigen Receptor
Cluster Analysis
Fluorescence
Blinking
Cell Surface Receptors
Spectrum Analysis
Ligands
T-Lymphocytes

ASJC Scopus subject areas

  • Biophysics

Cite this

Quantum dot fluorescence characterizes the nanoscale organization of T cell receptors for antigen. / Boyle, Sarah; Kolin, David L.; Bieler, Joan Glick; Schneck, Jonathan P; Wiseman, Paul W.; Edidin, Michael A.

In: Biophysical Journal, Vol. 101, No. 11, 07.12.2011.

Research output: Contribution to journalArticle

Boyle, Sarah ; Kolin, David L. ; Bieler, Joan Glick ; Schneck, Jonathan P ; Wiseman, Paul W. ; Edidin, Michael A. / Quantum dot fluorescence characterizes the nanoscale organization of T cell receptors for antigen. In: Biophysical Journal. 2011 ; Vol. 101, No. 11.
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