QUANTITY: An isobaric tag for quantitative glycomics

Shuang Yang, Meiyao Wang, Lijun Chen, Bojiao Yin, Guoqiang Song, Illarion V. Turko, Karen W. Phinney, Michael J. Betenbaugh, Hui Zhang, Shuwei Li

Research output: Contribution to journalArticlepeer-review

Abstract

Glycan is an important class of macromolecules that play numerous biological functions. Quantitative glycomics - analysis of glycans at global level - however, is far behind genomics and proteomics owing to technical challenges associated with their chemical properties and structural complexity. As a result, technologies that can facilitate global glycan analysis are highly sought after. Here, we present QUANTITY (Quaternary Amine Containing Isobaric Tag for Glycan), a quantitative approach that can not only enhance detection of glycans by mass spectrometry, but also allow high-throughput glycomic analysis from multiple biological samples. This robust tool enabled us to accomplish glycomic survey of bioengineered Chinese Hamster Ovary (CHO) cells with knock-in/out enzymes involved in protein glycosylation. Our results demonstrated QUANTITY is an invaluable technique for glycan analysis and bioengineering.

Original languageEnglish (US)
Article number17585
JournalScientific reports
Volume5
DOIs
StatePublished - Nov 30 2015

ASJC Scopus subject areas

  • General

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