Quantitative trait loci associated with maximal exercise endurance in mice

J. Timothy Lightfoot, Michael J. Turner, Amy Kleinfehn Knab, Anne E. Jedlicka, Tomohiro Oshimura, Jacqui Marzec, Wesley Gladwell, Larry J. Leamy, Steven R. Kleeberger

Research output: Contribution to journalArticlepeer-review

Abstract

The role of genetics in the determination of maximal exercise endurance is unclear. Six- to nine-week-old F2 mice (n = 99; 60 female, 39 male), derived from an intercross of two inbred strains that had previously been phenotyped as having high maximal exercise endurance (Balb/cJ) and low maximal exercise endurance (DBA/2J), were treadmill tested to estimate exercise endurance. Selective genotyping of the F2 cohort (n = 12 high exercise endurance; n = 12 low exercise endurance) identified a significant quantitative trait locus (QTL) on chromosome X (53.7 cM, DXMit121) in the entire cohort and a suggestive QTL on chromosome 8 (36.1 cM, D8Mit359) in the female mice. Fine mapping with the entire F2 cohort and additional informative markers confirmed and narrowed the QTLs. The chromosome 8 QTL (EE8F) is homologous with two suggestive human QTLs and one significant rat QTL previously linked with exercise endurance. No effect of sex (P = 0.33) or body weight (P = 0.79) on exercise endurance was found in the F2 cohort. These data indicate that genetic factors in distinct chromosomal regions may affect maximal exercise endurance in the inbred mouse. Whereas multiple genes are located in the identified QTL that could functionally affect exercise endurance, this study serves as a foundation for further investigations delineating the identity of genetic factors influencing maximum exercise endurance.

Original languageEnglish (US)
Pages (from-to)105-110
Number of pages6
JournalJournal of applied physiology
Volume103
Issue number1
DOIs
StatePublished - Jul 2007

Keywords

  • Aerobic capacity
  • Candidate genes
  • Genotyping

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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