Quantitative 1H NMR spectroscopy

Santosh Kumar Bharti; Raja Roy

doi:10.1016/j.trac.2012.02.007

Quantitative ¹H NMR spectroscopy

Santosh Kumar Bharti, Raja Roy

Research output: Contribution to journal › Review article › peer-review

353 Scopus citations

Abstract

This review illustrates the need to use nuclear magnetic resonance (NMR) spectroscopy for the quantitative analysis of small molecules in their crude forms and in mixtures.We provide the basic concepts of quantitative NMR (qNMR), a brief description of important acquisition and processing parameters responsible for obtaining high-quality, reproducible NMR spectra in order to maximize accuracy, and the latest referencing techniques used for quantitative analysis.We also describe methods that are used for quantitative analysis including calibration-curve and standard-addition methods. Further, we briefly address validation of qNMR spectroscopy and its major applications in various scientific disciplines.

Original language	English (US)
Pages (from-to)	5-26
Number of pages	22
Journal	TrAC - Trends in Analytical Chemistry
Volume	35
DOIs	https://doi.org/10.1016/j.trac.2012.02.007
State	Published - May 2012
Externally published	Yes

Keywords

Calibration-curve method
Electronic referencing
Metabolomics
NMR spectroscopy
Nuclear magnetic resonance (NMR)
Pharmaceutical analysis
Quantitative NMR (qNMR)
Referencing technique
Standard-addition method
Validation

ASJC Scopus subject areas

Analytical Chemistry
Spectroscopy

Access to Document

10.1016/j.trac.2012.02.007

Cite this

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title = "Quantitative 1H NMR spectroscopy",

abstract = "This review illustrates the need to use nuclear magnetic resonance (NMR) spectroscopy for the quantitative analysis of small molecules in their crude forms and in mixtures.We provide the basic concepts of quantitative NMR (qNMR), a brief description of important acquisition and processing parameters responsible for obtaining high-quality, reproducible NMR spectra in order to maximize accuracy, and the latest referencing techniques used for quantitative analysis.We also describe methods that are used for quantitative analysis including calibration-curve and standard-addition methods. Further, we briefly address validation of qNMR spectroscopy and its major applications in various scientific disciplines.",

keywords = "Calibration-curve method, Electronic referencing, Metabolomics, NMR spectroscopy, Nuclear magnetic resonance (NMR), Pharmaceutical analysis, Quantitative NMR (qNMR), Referencing technique, Standard-addition method, Validation",

author = "Bharti, {Santosh Kumar} and Raja Roy",

note = "Funding Information: Financial assistance from the Department of Science and Technology, Government of India, is gratefully acknowledged. ",

year = "2012",

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language = "English (US)",

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journal = "TrAC - Trends in Analytical Chemistry",

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AU - Bharti, Santosh Kumar

AU - Roy, Raja

N1 - Funding Information: Financial assistance from the Department of Science and Technology, Government of India, is gratefully acknowledged.

PY - 2012/5

Y1 - 2012/5

N2 - This review illustrates the need to use nuclear magnetic resonance (NMR) spectroscopy for the quantitative analysis of small molecules in their crude forms and in mixtures.We provide the basic concepts of quantitative NMR (qNMR), a brief description of important acquisition and processing parameters responsible for obtaining high-quality, reproducible NMR spectra in order to maximize accuracy, and the latest referencing techniques used for quantitative analysis.We also describe methods that are used for quantitative analysis including calibration-curve and standard-addition methods. Further, we briefly address validation of qNMR spectroscopy and its major applications in various scientific disciplines.

AB - This review illustrates the need to use nuclear magnetic resonance (NMR) spectroscopy for the quantitative analysis of small molecules in their crude forms and in mixtures.We provide the basic concepts of quantitative NMR (qNMR), a brief description of important acquisition and processing parameters responsible for obtaining high-quality, reproducible NMR spectra in order to maximize accuracy, and the latest referencing techniques used for quantitative analysis.We also describe methods that are used for quantitative analysis including calibration-curve and standard-addition methods. Further, we briefly address validation of qNMR spectroscopy and its major applications in various scientific disciplines.

KW - Calibration-curve method

KW - Electronic referencing

KW - Metabolomics

KW - NMR spectroscopy

KW - Nuclear magnetic resonance (NMR)

KW - Pharmaceutical analysis

KW - Quantitative NMR (qNMR)

KW - Referencing technique

KW - Standard-addition method

KW - Validation

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DO - 10.1016/j.trac.2012.02.007

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