Quantitative studies of toxin in the stools and jejunal aspirates of patients with cholera

K. M.S. Aziz, W. Henry Mosley

Research output: Contribution to journalArticlepeer-review

Abstract

Stools and jejunal aspirates from patients with acute cholera were sterilized by filtration and stored at —40 C to —50 C. These filtrates were assayed for potency in the skin of the back of adult rabbits (measured by blueing doses and limit of blueing units) and for fluid-producing activity in the ligated intestinal loops. Two toxins prepared in vitro were also assayed by the same techniques. The results showed a consistent relation among the three systems of assay for cholera toxin both from the in-vitro preparation, and from stools and jejunal aspirates. Based on assays of pure toxin, the content of toxin in stools from patients with acute cholera was estimated to be about 60 pg/liter. Stools had higher bacterial counts and were more toxic than jejunal aspirates obtained simultaneously from the same patients. There was no correlation between severity of disease, as judged by total output of stool, and the toxicity of the samples of stool or jejunal aspirates.

Original languageEnglish (US)
Pages (from-to)36-44
Number of pages9
JournalJournal of Infectious Diseases
Volume125
Issue number1
DOIs
StatePublished - Jan 1972
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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