TY - JOUR
T1 - Quantitative RARβ2 hypermethylation
T2 - A promising prostate cancer marker
AU - Jerónimo, Carmen
AU - Henrique, Rui
AU - Hoque, Mohammad O.
AU - Ribeiro, Franclim R.
AU - Oliveira, Jorge
AU - Fonseca, Daniel
AU - Teixeira, Manuel R.
AU - Lopes, Carlos
AU - Sidransky, David
PY - 2004/7/15
Y1 - 2004/7/15
N2 - Retinoic acid receptor β2 (RARβ2) is a tumor suppressor gene frequently hypermethylated in several human neoplasms. To further characterize this epigenetic alteration in prostate cancer progression, we examined tumor tissue from 118 patients with prostate carcinoma (PCa), 38 paired high-grade prostatic intraepithelial neoplasias (HGPIN, and non-neoplastic prostate tissue from 30 patients with benign prostate hyperplasia (BPH), using quantitative methylation-specific PCR. We found RARβ2 hypermethylation in 97.5% of PCa, 94.7% of HGPIN, and 23.3% of BPH. Methylation levels were significantly higher in PCa compared with HGPIN and BPH (P < 0.00001). By establishing an empiric cutoff value, we were able to discriminate between neoplastic and non-neoplastic tissue, with 94.9% sensitivity and 100% specificity. Moreover, RARβ2 methylation levels correlated with higher pathological stage (r = 0.30, P = 0.0009). This quantitative assay represents a novel and promising molecular marker that may augment current approaches for prostate cancer detection.
AB - Retinoic acid receptor β2 (RARβ2) is a tumor suppressor gene frequently hypermethylated in several human neoplasms. To further characterize this epigenetic alteration in prostate cancer progression, we examined tumor tissue from 118 patients with prostate carcinoma (PCa), 38 paired high-grade prostatic intraepithelial neoplasias (HGPIN, and non-neoplastic prostate tissue from 30 patients with benign prostate hyperplasia (BPH), using quantitative methylation-specific PCR. We found RARβ2 hypermethylation in 97.5% of PCa, 94.7% of HGPIN, and 23.3% of BPH. Methylation levels were significantly higher in PCa compared with HGPIN and BPH (P < 0.00001). By establishing an empiric cutoff value, we were able to discriminate between neoplastic and non-neoplastic tissue, with 94.9% sensitivity and 100% specificity. Moreover, RARβ2 methylation levels correlated with higher pathological stage (r = 0.30, P = 0.0009). This quantitative assay represents a novel and promising molecular marker that may augment current approaches for prostate cancer detection.
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U2 - 10.1158/1078-0432.CCR-03-0643
DO - 10.1158/1078-0432.CCR-03-0643
M3 - Article
C2 - 15217932
AN - SCOPUS:3042619078
SN - 1078-0432
VL - 10
SP - 4010
EP - 4014
JO - Clinical Cancer Research
JF - Clinical Cancer Research
IS - 12 I
ER -