Quantitative MRI volumetry, diffusivity, cerebrovascular flow, and cranial hydrodynamics during head-down tilt and hypercapnia: The SPACECOT study

SPACECOT Investigators Group

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


To improve the pathophysiological understanding of visual changes observed in astronauts, we aimed to use quantitative MRI to measure anatomic and physiological responses during a ground-based spaceflight analog (head-down tilt, HDT) combined with increased ambient carbon dioxide (CO2). Six healthy, male subjects participated in the double-blinded, randomized crossover design study with two conditions: 26.5 h of -12° HDT with ambient air and with 0.5% CO2, both followed by 2.5-h exposure to 3% CO2. Volume and mean diffusivity quantification of the lateral ventricle and phase-contrast flow sequences of the internal carotid arteries and cerebral aqueduct were acquired at 3 T. Compared with supine baseline, HDT (ambient air) resulted in an increase in lateral ventricular volume (P = 0.03). Cerebral blood flow, however, decreased with HDT in the presence of either ambient air or 0.5% CO2 (P = 0.002 and P = 0.01, respectively); this was partially reversed by acute 3% CO2 exposure. Following HDT (ambient air), exposure to 3% CO2 increased aqueductal cerebral spinal fluid velocity amplitude (P = 0.01) and lateral ventricle cerebrospinal fluid (CSF) mean diffusivity (P = 0.001). We concluded that HDT causes alterations in cranial anatomy and physiology that are associated with decreased craniospinal compliance. Brief exposure to 3% CO2 augments CSF pulsatility within the cerebral aqueduct and lateral ventricles. NEW & NOTEWORTHY: Head-down tilt causes increased lateral ventricular volume and decreased cerebrovascular flow after 26.5 h. Additional short exposure to 3% ambient carbon dioxide levels causes increased cerebrovascular flow associated with increased cerebrospinal fluid pulsatility at the cerebral aqueduct. Head-down tilt with chronically elevated 0.5% ambient carbon dioxide and acutely elevated 3% ambient carbon dioxide causes increased mean diffusivity of cerebral spinal fluid within the lateral ventricles.

Original languageEnglish (US)
Pages (from-to)1155-1166
Number of pages12
JournalJournal of applied physiology
Issue number5
StatePublished - May 2017


  • Headdown tilt
  • Hypercapnia
  • Intracranial hypertension
  • Microgravity
  • Space analog
  • dMRI

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)


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