Quantitative molecular profiling of biomarkers for pancreatic cancer with functionalized quantum dots

Kwan Hyi Lee, Justin F. Galloway, Jeaho Park, Charlene M. Dvoracek, Matthew Dallas, Konstaninos Konstantopoulos, Anirban Maitra, Peter C. Searson

Research output: Contribution to journalArticle

Abstract

Applications in nanomedicine, such as diagnostics and targeted therapeutics, rely on the detection and targeting of membrane biomarkers. In this article we demonstrate absolute quantitative profiling, spatial mapping, and multiplexing of cancer biomarkers using functionalized quantum dots (QDs). We demonstrate highly selective targeting molecular markers for pancreatic cancer with extremely low levels of nonspecific binding. We confirm that we have saturated all biomarkers on the cell surface, and, in conjunction with control experiments, extract absolute quantitative values for the biomarker density in terms of the number of molecules per square micron on the cell surface. We show that we can obtain quantitative spatial information of biomarker distribution on a single cell, important because tumors' cell populations are inherently heterogeneous. We validate our quantitative measurements (number of molecules per square micron) using flow cytometry and demonstrate multiplexed quantitative profiling using color-coded QDs. From the Clinical Editor: This paper demonstrates a nice example for quantum dot-based molecular targeting of pancreatic cancer cells for advanced high sensitivity diagnostics and potential future selective therapeutic purposes.

Original languageEnglish (US)
Pages (from-to)1043-1051
Number of pages9
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume8
Issue number7
DOIs
StatePublished - Oct 2012

Keywords

  • Biomarker profiling
  • Cancer biomarkers
  • Core/shell nanoparticles
  • Lipid encapsulation
  • Quantum dots

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

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