Quantitative measurements of protein interactions in a crowded cellular environment

Edwin Li, Jesse Placone, Mikhail Merzlyakov, Kalina Hristova

Research output: Contribution to journalArticlepeer-review

Abstract

Quantitative measurements of protein interaction strengths, crucial for describing signaling networks and predicting cellular responses to environmental stimuli, are typically performed in dilute buffer solutions. However, protein-protein interactions in cells occur within the context of a crowded system, which is characterized by a high macromolecular concentration. In this paper, we explore the utility of cell-derived vesicles as a model crowded environment for quantitative FRET measurements of protein-protein interactions. We show that the FRET efficiency, and the donor and acceptor concentrations, can be calculated in each vesicle. We also introduce the "quantitative imaging Foster resonance energy transfer" method as a tool that can yield protein interaction strengths within these vesicles.

Original languageEnglish (US)
Pages (from-to)5976-5985
Number of pages10
JournalAnalytical chemistry
Volume80
Issue number15
DOIs
StatePublished - Aug 1 2008

ASJC Scopus subject areas

  • Analytical Chemistry

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