TY - JOUR
T1 - Quantitative measurement of nonisotopically labeled polymerase chain reaction product
AU - Yang, Bingzhi
AU - Viscidi, Raphael
AU - Yolken, Robert
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1993/9
Y1 - 1993/9
N2 - A novel approach for evaluation of polymerase chain reaction product was described. A biotin-labeled DNA (or RNA) probe is hybridized to the digoxigenin-labeled target PCR product. The hybrids are retained on an anti-biotin antibody-coated plate. An anti-digoxigenin Fab conjugated with alkaline phosphatase is used for generating either a fluorescence or a chromagenic substance. Both fluorogenic and chromagenic systems can be used for quantitative measurement of the PCR product/probe hybrids. The method is specific, fast, nonisotopic, sensitive, quantitative, and practical.
AB - A novel approach for evaluation of polymerase chain reaction product was described. A biotin-labeled DNA (or RNA) probe is hybridized to the digoxigenin-labeled target PCR product. The hybrids are retained on an anti-biotin antibody-coated plate. An anti-digoxigenin Fab conjugated with alkaline phosphatase is used for generating either a fluorescence or a chromagenic substance. Both fluorogenic and chromagenic systems can be used for quantitative measurement of the PCR product/probe hybrids. The method is specific, fast, nonisotopic, sensitive, quantitative, and practical.
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U2 - 10.1006/abio.1993.1441
DO - 10.1006/abio.1993.1441
M3 - Article
C2 - 8238919
AN - SCOPUS:0027220987
SN - 0003-2697
VL - 213
SP - 422
EP - 425
JO - Analytical biochemistry
JF - Analytical biochemistry
IS - 2
ER -