Quantitative measurement of nonisotopically labeled polymerase chain reaction product

Research output: Contribution to journalArticle

Abstract

A novel approach for evaluation of polymerase chain reaction product was described. A biotin-labeled DNA (or RNA) probe is hybridized to the digoxigenin-labeled target PCR product. The hybrids are retained on an anti-biotin antibody-coated plate. An anti-digoxigenin Fab conjugated with alkaline phosphatase is used for generating either a fluorescence or a chromagenic substance. Both fluorogenic and chromagenic systems can be used for quantitative measurement of the PCR product/probe hybrids. The method is specific, fast, nonisotopic, sensitive, quantitative, and practical.

Original languageEnglish (US)
Pages (from-to)422-425
Number of pages4
JournalAnalytical biochemistry
Volume213
Issue number2
DOIs
StatePublished - Sep 1993

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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