Quantitative localization of [3H]TCP binding in rat brain by light microscopy autoradiography

Ratna Sircar, Stephen R. Zukin

Research output: Contribution to journalArticle

Abstract

The anatomical localization of phencyclidine (PCP)/σ-opiate receptors in rat brain was determined by quantitative light microscopy autoradiography using the new ligand N-(1-[2-thienyl]cyclohexyl)[3H]TCP). TCP is a potent analog of PCP which possesses a higher affinity for PCP/σ-opiate receptor than those PCP itself. The higest of [3H]TCP binding was detected in the hippocampus. Intermediate levels were found in frontal cortex, striatum, amygdala and cerebellum. Specific [3H]TCP binding was undetectable in anterior commissure and corpus callosum. The distribution pattern of [3H]TCP binding sites is similar to the pattern obtained with [3H]PCP but more sharply defined. On the basis of its greater potency and specificity, [3H]TCP may prove superior to [3H]PCP as a molecular probe for the study of brain sigma opiate/phencyclidine receptors.

Original languageEnglish (US)
Pages (from-to)142-145
Number of pages4
JournalBrain Research
Volume344
Issue number1
DOIs
Publication statusPublished - Sep 30 1985
Externally publishedYes

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Keywords

  • autoradiography
  • N-(1-[2-thienyl]cyclohexyl)[H]piperidine ([H]TCP)
  • phencyclidine (PCP)/σ-opiate receptor
  • phencyclidine analog
  • quantitative light microscopy

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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