Quantitative determination of methylated nucleosides and pseudouridine in urine by gas-liquid chromatography

Sai Y. Chang, Duane B. Lakings, Robert W. Zumwalt, Charles W. Gehrke, T. Phillip Waalkes

Research output: Contribution to journalArticle

Abstract

Evidence has been presented indicating that specific nucleosides, N2,N2-dimethylguanosine and 1-methylinosine, and pseudouridine, degradation products of transfer ribonucleic acid, may be elevated in the urine of patients with cancer. Previous methods of analysis lacked the sensitivity and specificity, and were too tedious for practical application. In order to study the urinary excretion of these nucleosides a sensitive and rapid gas-liquid chromatographic method has been developed. Following a charcoal adsorption cleanup procedure and elution with pyridine-ethanol solution, the nucleosides were determined by gas-liquid chromatography as their trimethylsilyl derivatives. Studies were carried out to establish the derivatization conditions, precision, and recovery of the nucleosides in urine. Application of the method has been shown by determining the excretion levels of the nucleosides in urine of normal control subjects.

Original languageEnglish (US)
Pages (from-to)816-830
Number of pages15
JournalThe Journal of laboratory and clinical medicine
Volume83
Issue number5
StatePublished - May 1974

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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