Quantitative determination of methylated nucleosides and pseudouridine in urine by gas-liquid chromatography

Sai Y. Chang, Duane B. Lakings, Robert W. Zumwalt, Charles W. Gehrke, T. Phillip Waalkes

Research output: Contribution to journalArticle

Abstract

Evidence has been presented indicating that specific nucleosides, N2,N2-dimethylguanosine and 1-methylinosine, and pseudouridine, degradation products of transfer ribonucleic acid, may be elevated in the urine of patients with cancer. Previous methods of analysis lacked the sensitivity and specificity, and were too tedious for practical application. In order to study the urinary excretion of these nucleosides a sensitive and rapid gas-liquid chromatographic method has been developed. Following a charcoal adsorption cleanup procedure and elution with pyridine-ethanol solution, the nucleosides were determined by gas-liquid chromatography as their trimethylsilyl derivatives. Studies were carried out to establish the derivatization conditions, precision, and recovery of the nucleosides in urine. Application of the method has been shown by determining the excretion levels of the nucleosides in urine of normal control subjects.

Original languageEnglish (US)
Pages (from-to)816-830
Number of pages15
JournalThe Journal of Laboratory and Clinical Medicine
Volume83
Issue number5
StatePublished - 1974
Externally publishedYes

Fingerprint

Pseudouridine
Liquid chromatography
Nucleosides
Gas chromatography
Gas Chromatography
Urine
Charcoal
Transfer RNA
Adsorption
Ethanol
Gases
Derivatives
Sensitivity and Specificity
Recovery
Degradation
Liquids
Neoplasms

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

Chang, S. Y., Lakings, D. B., Zumwalt, R. W., Gehrke, C. W., & Waalkes, T. P. (1974). Quantitative determination of methylated nucleosides and pseudouridine in urine by gas-liquid chromatography. The Journal of Laboratory and Clinical Medicine, 83(5), 816-830.

Quantitative determination of methylated nucleosides and pseudouridine in urine by gas-liquid chromatography. / Chang, Sai Y.; Lakings, Duane B.; Zumwalt, Robert W.; Gehrke, Charles W.; Waalkes, T. Phillip.

In: The Journal of Laboratory and Clinical Medicine, Vol. 83, No. 5, 1974, p. 816-830.

Research output: Contribution to journalArticle

Chang, Sai Y. ; Lakings, Duane B. ; Zumwalt, Robert W. ; Gehrke, Charles W. ; Waalkes, T. Phillip. / Quantitative determination of methylated nucleosides and pseudouridine in urine by gas-liquid chromatography. In: The Journal of Laboratory and Clinical Medicine. 1974 ; Vol. 83, No. 5. pp. 816-830.
@article{f912f410d3994143a3dabd4fe54d68ce,
title = "Quantitative determination of methylated nucleosides and pseudouridine in urine by gas-liquid chromatography",
abstract = "Evidence has been presented indicating that specific nucleosides, N2,N2-dimethylguanosine and 1-methylinosine, and pseudouridine, degradation products of transfer ribonucleic acid, may be elevated in the urine of patients with cancer. Previous methods of analysis lacked the sensitivity and specificity, and were too tedious for practical application. In order to study the urinary excretion of these nucleosides a sensitive and rapid gas-liquid chromatographic method has been developed. Following a charcoal adsorption cleanup procedure and elution with pyridine-ethanol solution, the nucleosides were determined by gas-liquid chromatography as their trimethylsilyl derivatives. Studies were carried out to establish the derivatization conditions, precision, and recovery of the nucleosides in urine. Application of the method has been shown by determining the excretion levels of the nucleosides in urine of normal control subjects.",
author = "Chang, {Sai Y.} and Lakings, {Duane B.} and Zumwalt, {Robert W.} and Gehrke, {Charles W.} and Waalkes, {T. Phillip}",
year = "1974",
language = "English (US)",
volume = "83",
pages = "816--830",
journal = "Translational Research",
issn = "1931-5244",
publisher = "Mosby Inc.",
number = "5",

}

TY - JOUR

T1 - Quantitative determination of methylated nucleosides and pseudouridine in urine by gas-liquid chromatography

AU - Chang, Sai Y.

AU - Lakings, Duane B.

AU - Zumwalt, Robert W.

AU - Gehrke, Charles W.

AU - Waalkes, T. Phillip

PY - 1974

Y1 - 1974

N2 - Evidence has been presented indicating that specific nucleosides, N2,N2-dimethylguanosine and 1-methylinosine, and pseudouridine, degradation products of transfer ribonucleic acid, may be elevated in the urine of patients with cancer. Previous methods of analysis lacked the sensitivity and specificity, and were too tedious for practical application. In order to study the urinary excretion of these nucleosides a sensitive and rapid gas-liquid chromatographic method has been developed. Following a charcoal adsorption cleanup procedure and elution with pyridine-ethanol solution, the nucleosides were determined by gas-liquid chromatography as their trimethylsilyl derivatives. Studies were carried out to establish the derivatization conditions, precision, and recovery of the nucleosides in urine. Application of the method has been shown by determining the excretion levels of the nucleosides in urine of normal control subjects.

AB - Evidence has been presented indicating that specific nucleosides, N2,N2-dimethylguanosine and 1-methylinosine, and pseudouridine, degradation products of transfer ribonucleic acid, may be elevated in the urine of patients with cancer. Previous methods of analysis lacked the sensitivity and specificity, and were too tedious for practical application. In order to study the urinary excretion of these nucleosides a sensitive and rapid gas-liquid chromatographic method has been developed. Following a charcoal adsorption cleanup procedure and elution with pyridine-ethanol solution, the nucleosides were determined by gas-liquid chromatography as their trimethylsilyl derivatives. Studies were carried out to establish the derivatization conditions, precision, and recovery of the nucleosides in urine. Application of the method has been shown by determining the excretion levels of the nucleosides in urine of normal control subjects.

UR - http://www.scopus.com/inward/record.url?scp=0016065908&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016065908&partnerID=8YFLogxK

M3 - Article

C2 - 4821860

AN - SCOPUS:0016065908

VL - 83

SP - 816

EP - 830

JO - Translational Research

JF - Translational Research

SN - 1931-5244

IS - 5

ER -