Quantitative detection of multiple fluorophore sites as a tool for diagnosis and monitoring disease progression in salivary glands

Israel Gannot, Robert F. Bonner, Gallya Gannot, Philip C. Fox, Joon S. You, Ronald W. Waynant, Amir H. Gandjbakhche

Research output: Contribution to journalConference article

Abstract

A series of fluorescent surface images were obtained from physical models of localized fluorophores embedded at various depths and separations in tissue phantoms. Our random walk theory was applied to create an analytical model of multiple flurophores embedded in tissue-like phantom. Using this model, From accuired set of surface images, the location of the fluorophores was reconstructed and compared it to their known 3-D distributions. A good correlation was found, and the ability to resolve fluorophores as a function of depth and separation was determined. In paralel in in-vitro study, specific coloring of sections of minor salivary glands was also demonstrated. These results demonstrate the possibility of using inverse methods to reconstruct unknown locations and concentrations of optical probes specifically bound to infiltrating lymphocytes in minor salivary glands of patients with Sjögren's syndrome.

Original languageEnglish (US)
Pages (from-to)151-156
Number of pages6
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume2979
DOIs
StatePublished - Dec 1 1997
Externally publishedYes
EventProceedings of Optical Tomography and Spectroscopy of Tissue: Theory, Instrumentation, Model and Human Studies II - San Jose, CA, United States
Duration: Feb 9 1997Feb 12 1997

Fingerprint

salivary glands
Fluorophores
Phantom
Progression
progressions
Minor
Monitoring
Inverse Method
Lymphocytes
Physical Model
Analytical Model
Tissue
3D
Colouring
Random walk
Resolve
Probe
lymphocytes
Coloring
random walk

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Quantitative detection of multiple fluorophore sites as a tool for diagnosis and monitoring disease progression in salivary glands. / Gannot, Israel; Bonner, Robert F.; Gannot, Gallya; Fox, Philip C.; You, Joon S.; Waynant, Ronald W.; Gandjbakhche, Amir H.

In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 2979, 01.12.1997, p. 151-156.

Research output: Contribution to journalConference article

Gannot, Israel ; Bonner, Robert F. ; Gannot, Gallya ; Fox, Philip C. ; You, Joon S. ; Waynant, Ronald W. ; Gandjbakhche, Amir H. / Quantitative detection of multiple fluorophore sites as a tool for diagnosis and monitoring disease progression in salivary glands. In: Proceedings of SPIE - The International Society for Optical Engineering. 1997 ; Vol. 2979. pp. 151-156.
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