Quantitative cytophotometric analysis of brain neuronal RNA and protein changes in acute T-2 mycotoxin poisoned rats

Lee J Martin, J. D. Morse, A. Anthony

Research output: Contribution to journalArticle

Abstract

L. J. Martin, J. D. Morse and A. Anthony. Quantitative cytophotometric analysis of brain neuronal RNA and protein changes in acute T-2 mycotoxin poisoned rats. Toxicon 24, 933 - 941, 1986.-Male Sprague-Dawley rats (200 g) injected intraperitoneally with T-2 toxin, a trichothecene mycotoxin protein synthesis inhibitor, at dosages of 0.75, 1.0, 1.5 and 6.0 mg/kg (1 LD50 = 0.9 mg/kg) were decapitated at 8 hr post-exposure. Data were obtained on changes in neuronal (perikaryal) RNA levels, protein contents and nucleolar volumes in cerebrocortical (layer III) and striatal (caudate - putamen) brain regions using quantitative azure B - RNA and Coomassie - protein cytophotometry and ocular filar micrometry. Correlative observations were made on changes in brain cytomorphology. Reductions in neuronal RNA/protein contents and nucleolar volume were used as indices of impaired perikaryal functioning. At 8 hr after T-2 toxin poisoning the following results were obtained in cerebrocortical and striatal brain compartments: (1) neuronal RNA contents were generally maintained at control values in both brain regions, however, moderate RNA depletion was evidenced in the cerebral cortex with 1.5 mg/kg T-2 and in the striatum with a 6.0 mg/kg dose; (2) neuronal protein levels were suppressed in a dose-dependent fashion within the cerebrocortex, while in the striatum there was no direct correspondence between protein loss and T-2 dosage; (3) neuronal nucleolar volumes were typically maintained at control levels in both neuronal compartments. Microscopic observations revealed no gross evidence of T-2-induced brain cytopathology. These data indicate that T-2 toxin does not elicit direct cytopathic actions in these two brain regions, thus indicating that cerebrocortical and striatal compartments do not represent primary target sites of T-2 toxicant action. It is believed that neuronal protein depletion in these brain regions is more likely attributable to systemic effects of T-2 mycotoxin leading to cardiovascular insufficiency and hypoxemia.

Original languageEnglish (US)
Pages (from-to)933-941
Number of pages9
JournalToxicon
Volume24
Issue number9
DOIs
StatePublished - 1986
Externally publishedYes

Fingerprint

Mycotoxins
Rats
Brain
RNA
T-2 Toxin
Chemical analysis
Corpus Striatum
Proteins
Nuclear Proteins
Trichothecenes
Cytophotometry
Protein Synthesis Inhibitors
Putamen
Lethal Dose 50
Level control
Cerebral Cortex
Poisoning
Sprague Dawley Rats

ASJC Scopus subject areas

  • Toxicology

Cite this

Quantitative cytophotometric analysis of brain neuronal RNA and protein changes in acute T-2 mycotoxin poisoned rats. / Martin, Lee J; Morse, J. D.; Anthony, A.

In: Toxicon, Vol. 24, No. 9, 1986, p. 933-941.

Research output: Contribution to journalArticle

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