Quantitative CT and 19 F-MRI tracking of perfluorinated encapsulated mesenchymal stem cells to assess graft immunorejection

Guan Wang, Yingli Fu, Steven M. Shea, Shashank Sathyanarayana Hegde, Dara L. Kraitchman

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives: Peripheral artery disease (PAD) affects 12–14% of the world population, and many are not eligible for conventional treatment. For these patients, microencapsulated stem cells (SCs) offer a novel means to transplant mismatched therapeutic SCs to prevent graft immunorejection. Using c-arm CT and 19 F-MRI for serial evaluation of dual X-ray/MR-visible SC microcapsules (XMRCaps) in a non-immunosuppressed rabbit PAD model, we explore quantitative evaluation of capsule integrity as a surrogate of transplanted cell fate. Materials and methods: XMRCaps were produced by impregnating 12% perfluorooctylbromine (PFOB) with rabbit or human SCs (AlloSC and XenoSC, respectively). Volume and 19 F concentration measurements of XMRCaps were assessed both in phantoms and in vivo, at days 1, 8 and 15 after intramuscular administration in rabbits (n = 10), by 3D segmenting the injection sites and referencing to standards with known concentrations. Results: XMRCap volumes and concentrations showed good agreement between CT and MRI both in vitro and in vivo in XenoSC rabbits. Injected capsules showed small variations over time and were similar between AlloSC and XenoSC rabbits. Histological staining revealed high cell viability and intact capsules 2 weeks after administration. Conclusions: Quantitative and non-invasive tracking XMRCaps using CT and 19 F-MRI may be useful to assess graft immunorejection after SC transplantation.

Original languageEnglish (US)
Pages (from-to)147-156
Number of pages10
JournalMagnetic Resonance Materials in Physics, Biology and Medicine
Volume32
Issue number1
DOIs
StatePublished - Feb 14 2019

Keywords

  • Encapsulated stem cells
  • F-MRI
  • Immunorejection
  • Peripheral artery disease
  • Quantitative tracking

ASJC Scopus subject areas

  • Biophysics
  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

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