Quantitative autoradiography of [3H]forskolin binding sites in the rat brain

D. R. Gehlert, T. M. Dawson, H. I. Yamamura, J. K. Wamsley

Research output: Contribution to journalArticlepeer-review

Abstract

The binding sites for a radiolabeled form of the potent activator of adenylate cyclase, forskolin, have been localized in the rat brain, pituitary and spinal cord. Using the quantitative technique of in vitro autoradiography, a high density of [3H]forskolin binding was detected in brain structures such as the caudate-putamen, nucleus accumbens, olfactory tubercle, globus pallidus, substantia nigra and the hilus of the area dentata. A comparison of the distribution of [3H]forskolin binding sites with those reported for several neurotransmitter receptor types indicated that forskolin identified adenylate cyclase was probably not linked to any single type of neurotransmitter receptor. These results also presented several new brain areas in which to investigate the neuronal role of adenylate cyclase.

Original languageEnglish (US)
Pages (from-to)351-360
Number of pages10
JournalBrain research
Volume361
Issue number1-2
DOIs
StatePublished - Dec 30 1985
Externally publishedYes

Keywords

  • adenylate cyclase
  • autoradiography
  • brain
  • forskolin

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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