Quantitative analysis of the change of metabolite fluxes along the pentose phosphate and glycolytic pathways in Tetrahymena in response to carbohydrates

Michael J Borowitz, R. B. Stein, J. J. Blum

Research output: Contribution to journalArticle

Abstract

A metabolic scheme of glycolysis and the pentose phosphate pathway has been constructed, assuming that the reactions occur in a single compartment. From this scheme, equations are written for a system in metabolic and isotopic steady state. These allow computation of the specific activity of every carbon atom of all the intermediates of the glycolytic and pentose phosphate pathways and consequently of the flux of carbon along each step of these pathways. A sufficiently large number of well distributed measurements of incorporation of radioactive label from different positions of several substrates into intermediates or products must be made to determine all the fluxes. This is done by choosing a set of metabolic fluxes, calculating incorporation with the aid of a computer, and then manipulating the flux rates until the computed incorporations match the data. The model is used in this paper to analyze the metabolism of the protozoan Tetrahymena pyriformis. The metabolic scheme of the model is consistent with all available information on the enzyme complement of this ciliate.

Original languageEnglish (US)
Pages (from-to)1589-1605
Number of pages17
JournalJournal of Biological Chemistry
Volume252
Issue number5
StatePublished - 1977
Externally publishedYes

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Pentoses
Tetrahymena
Pentose Phosphate Pathway
Metabolites
Phosphates
Carbohydrates
Tetrahymena pyriformis
Fluxes
Carbon Cycle
Glycolysis
Chemical analysis
Carbon
Enzymes
Metabolism
Labels
Atoms
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

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abstract = "A metabolic scheme of glycolysis and the pentose phosphate pathway has been constructed, assuming that the reactions occur in a single compartment. From this scheme, equations are written for a system in metabolic and isotopic steady state. These allow computation of the specific activity of every carbon atom of all the intermediates of the glycolytic and pentose phosphate pathways and consequently of the flux of carbon along each step of these pathways. A sufficiently large number of well distributed measurements of incorporation of radioactive label from different positions of several substrates into intermediates or products must be made to determine all the fluxes. This is done by choosing a set of metabolic fluxes, calculating incorporation with the aid of a computer, and then manipulating the flux rates until the computed incorporations match the data. The model is used in this paper to analyze the metabolism of the protozoan Tetrahymena pyriformis. The metabolic scheme of the model is consistent with all available information on the enzyme complement of this ciliate.",
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AU - Stein, R. B.

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