Quantitative analysis of human immunodeficiency virus type 1 antibody reactivity by western immunoblots: Evaluation of relative antibody levels in seropositive individuals and mothers

Rebeca B. Geffin, Shenghan Lai, Cecelia Hutto, Gwendolyn B. Scott, Walter A. Scott, Manny Master, Wade P. Parks

Research output: Contribution to journalArticle

Abstract

A quantitative analysis of antibody responses to human immunodeficiency virus type 1 (HIV- 1) proteins using Western immunoblots and 125I-labeled protein A is reproducible and can be validated. The antibody levels obtained by Western immunoblots were compared with stoichiometric p24 radioimmunoassay over a wide range of antibody (correlation coefficient.,94; P <.001). Antibody levels to gpl60 and gpl20 were validated using purified antigens. Analysis of antibody levels from 31 seropositive individuals revealed a statistically significant correlation between antibody levels to p24 and the other viral proteins except gpl20. Anti-gag p24 antibody was strongly correlated with antibodies to other env products, specifically gp41 and gpl60. Using the validated assay, HIV-l-infected mothers of infants were found to have highly variable levels of antibody to all viral proteins. Mothers of infected infants did not differ significantly from mothers of uninfected infants in antibody pattern or levels to any viral protein including gpl20.

Original languageEnglish (US)
Pages (from-to)111-118
Number of pages8
JournalJournal of Infectious Diseases
Volume165
Issue number1
DOIs
StatePublished - Jan 1992
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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