Quantitative analysis of cyclic AMP waves mediating aggregation in Dictyostelium discoideum

Peter N. Devreotes, Michael J. Potel, Stephen A. MacKay

Research output: Contribution to journalArticlepeer-review

Abstract

We have previously reported the detection of cAMP waves within monolayers of aggregating Dictyostelium discoideum cells (K. J. Tomchik and P. N. Devreotes, 1981, Science 212, 443-446). The computer-assisted analysis presented here of the fluorographic images of the cAMP waves reveals (1) all the waves have a consistent width and height; (2) cAMP concentrations within centers of concentric aggregation territories oscillate periodically while at spiral centers the concentration builds up to a plateau value within 2 mm; (3) cells within the region of intersection of two oppositely directed cAMP waves are stimulated to produce more cAMP than those responding to a single wave; (4) cells start to move when the cAMP level begins to increase and cease movement when the peak cAMP concentration reaches the cell.

Original languageEnglish (US)
Pages (from-to)405-415
Number of pages11
JournalDevelopmental biology
Volume96
Issue number2
DOIs
StatePublished - Apr 1983

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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