Quantitation of vaginally administered nonoxynol-9 in premenopausal women

Patricia Barditch-Crovo, Frank R Witter, Fayez Hamzeh, Jason McPherson, Pamela Stratton, Nancy J. Alexander, Carol Braun Trapnell

Research output: Contribution to journalArticle


A feasibility study was performed in 11 healthy nonpregnant premenopausal women to determine a method for collection and recovery of vaginally administered nonoxynol-9. We also determined if nonoxynol-9 could be quantitated in vaginal lavage fluid obtained 2 h after instillation of a standard precoital dose of a foam formulation of nonoxynol-9. Samples were analyzed in batch using a validated normal phase high-performance liquid chromatography (HPLC) method. Two hours after instillation of one dose of Delfen® Contraceptive Foam (100 mg), the quantity of nonoxynol-9 collected ranged from 10.8 to 67.8 mg (mean: 35.4 mg). This corresponds to a recovery of 11-70% of the administered dose. Quantitation of vaginally administered nonoxynol-9 is both practical and feasible. These data represent a critical first step in the evaluation of the safety and effectiveness of nonoxynol-9- containing products in the prevention of sexually transmitted diseases.

Original languageEnglish (US)
Pages (from-to)261-263
Number of pages3
Issue number4
Publication statusPublished - Apr 1997



  • HIV prevention
  • Nonoxynol-9
  • Spermicide
  • STD prevention

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Barditch-Crovo, P., Witter, F. R., Hamzeh, F., McPherson, J., Stratton, P., Alexander, N. J., & Trapnell, C. B. (1997). Quantitation of vaginally administered nonoxynol-9 in premenopausal women. Contraception, 55(4), 261-263. https://doi.org/10.1016/S0010-7824(97)00003-6