TY - JOUR
T1 - Quantitation of individual proteins by a two-stage electrophoresis procedure
AU - Ruddell, Alanna
AU - Jacobs-Lorena, Marcelo
N1 - Funding Information:
’ This work was supported by NIH Grant GM 25327-03 to M.J.L. A.R. was supported by Training Grant GM 07225-06. * Abbreviations used: SDS, sodium dodecyl sulfate; 2D, two dimensionak TCA, trichloroacetic acid; IEF, isoelectric focusing; p1, isoelectric point.
PY - 1982/5/15
Y1 - 1982/5/15
N2 - A technique has been developed for quantitating specific proteins present in a complex mixture, using a two-stage electrophoresis procedure. It consists of homogenizing a relevant portion of an isoelectric focusing gel into a slot of sodium dodecyl sulfate-polyacrylamide slab gel. After electrophoresis, proteins form bands which can be stained and analyzed by standard densitometric procedures. Radioactively labeled marker protein is used to monitor recovery, so that accurate quantitative measurements can be obtained. This method has been used to measure the abundance of actin in Drosophila egg chambers. The technique is applicable to the quantitation of many specific proteins among complex protein populations.
AB - A technique has been developed for quantitating specific proteins present in a complex mixture, using a two-stage electrophoresis procedure. It consists of homogenizing a relevant portion of an isoelectric focusing gel into a slot of sodium dodecyl sulfate-polyacrylamide slab gel. After electrophoresis, proteins form bands which can be stained and analyzed by standard densitometric procedures. Radioactively labeled marker protein is used to monitor recovery, so that accurate quantitative measurements can be obtained. This method has been used to measure the abundance of actin in Drosophila egg chambers. The technique is applicable to the quantitation of many specific proteins among complex protein populations.
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U2 - 10.1016/0003-2697(82)90276-7
DO - 10.1016/0003-2697(82)90276-7
M3 - Article
C2 - 6810722
AN - SCOPUS:0019955040
SN - 0003-2697
VL - 122
SP - 248
EP - 252
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -