Quantitation of hemoglobins within individual red cells: Asynchronous biosynthesis of fetal and adult hemoglobin during erythroid maturation in normal subjects

George J Dover, S. H. Boyer

Research output: Contribution to journalArticle

Abstract

We outline a method for estimating either HbF or HbA content in single erythrocytes and their precursors. Our method depends on microphotometric assay of darkfield reflectance arising from individual pericellular immunoprecipitates developed with anti-HbF or anti-HbA. When uniform-diameter latex microspheres were used to normalize comparisons between preparations, mean coefficient of variation for HbF reflectance among separate preparations of the same sample was

Original languageEnglish (US)
Pages (from-to)1082-1091
Number of pages10
JournalBlood
Volume56
Issue number6
StatePublished - 1980

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Fetal Hemoglobin
Biosynthesis
Hemoglobins
Cells
Latex
Microspheres
Assays
Erythrocytes

ASJC Scopus subject areas

  • Hematology

Cite this

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abstract = "We outline a method for estimating either HbF or HbA content in single erythrocytes and their precursors. Our method depends on microphotometric assay of darkfield reflectance arising from individual pericellular immunoprecipitates developed with anti-HbF or anti-HbA. When uniform-diameter latex microspheres were used to normalize comparisons between preparations, mean coefficient of variation for HbF reflectance among separate preparations of the same sample was",
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