Quantitation of free radical-mediated reperfusion injury in renal transplantation

G. Melville Williams

Research output: Contribution to journalArticle

Abstract

This chapter discusses the quantitation of free radical-mediated reperfusion injury in renal transplantation. Schneeberger reported on 100 cadaver donor kidneys in which one of the pair of kidneys was treated with superoxide dismutase (SOD) at reperfusion. The study was randomized and double-blinded. Considering the data overall, there were no significant differences in the function of SOD-and placebo-treated kidneys. However, more detailed analysis of the pairs revealed that SOD-treated kidneys had double the creatinine clearance at days 2 and 7, and the time for serum creatinine to fall without dialysis was reduced from 8 to 4 days. Because of small numbers, these differences did not prove to be statistically significant. Considering the function of the second of the two kidneys of a pair transplanted, by selecting kidneys that were ischemic in the cold for longer periods of time, more dramatic improvement was noted in all parameters in test kidneys, but the differences still fell short of statistical significance. Thus, there is no conclusive data in humans documenting a window of reperfusion damage. However, the existing data are encouraging enough to promote two larger studies.

Original languageEnglish (US)
Pages (from-to)748-751
Number of pages4
JournalMethods in enzymology
Volume186
Issue numberC
DOIs
StatePublished - Jan 1 1990

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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