Quantifying focal disruptions in axonal microtubules

Raha Dastgheyb, K. A. Barbee, G. Gallo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Focal disruptions in axonal microtubules are a common consequence of Traumatic Brain Injuries (TBIs), and the ability to quantify these disruptions is essential to evaluating in vitro injury models and therapies. Here, we develop and implement an algorithm to demonstrate the ability to quantify focal disruptions in the microtubules of cultured neurons. The algorithm is implemented using a combination of ImageJ for pre-processing and Matlab for axon analysis and tubulin breakdown identification.

Original languageEnglish (US)
Title of host publication2015 41st Annual Northeast Biomedical Engineering Conference, NEBEC 2015
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781479983605
DOIs
StatePublished - Jun 2 2015
Externally publishedYes
Event2015 41st Annual Northeast Biomedical Engineering Conference, NEBEC 2015 - Troy, United States
Duration: Apr 17 2015Apr 19 2015

Publication series

Name2015 41st Annual Northeast Biomedical Engineering Conference, NEBEC 2015

Other

Other2015 41st Annual Northeast Biomedical Engineering Conference, NEBEC 2015
CountryUnited States
CityTroy
Period4/17/154/19/15

Fingerprint

Microtubules
Tubulin
Neurons
Axons
Brain
Wounds and Injuries
Processing
Therapeutics
Traumatic Brain Injury
In Vitro Techniques

ASJC Scopus subject areas

  • Biotechnology
  • Cancer Research
  • Cell Biology
  • Molecular Medicine
  • Biomedical Engineering
  • Control and Systems Engineering

Cite this

Dastgheyb, R., Barbee, K. A., & Gallo, G. (2015). Quantifying focal disruptions in axonal microtubules. In 2015 41st Annual Northeast Biomedical Engineering Conference, NEBEC 2015 [7117155] (2015 41st Annual Northeast Biomedical Engineering Conference, NEBEC 2015). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NEBEC.2015.7117155

Quantifying focal disruptions in axonal microtubules. / Dastgheyb, Raha; Barbee, K. A.; Gallo, G.

2015 41st Annual Northeast Biomedical Engineering Conference, NEBEC 2015. Institute of Electrical and Electronics Engineers Inc., 2015. 7117155 (2015 41st Annual Northeast Biomedical Engineering Conference, NEBEC 2015).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Dastgheyb, R, Barbee, KA & Gallo, G 2015, Quantifying focal disruptions in axonal microtubules. in 2015 41st Annual Northeast Biomedical Engineering Conference, NEBEC 2015., 7117155, 2015 41st Annual Northeast Biomedical Engineering Conference, NEBEC 2015, Institute of Electrical and Electronics Engineers Inc., 2015 41st Annual Northeast Biomedical Engineering Conference, NEBEC 2015, Troy, United States, 4/17/15. https://doi.org/10.1109/NEBEC.2015.7117155
Dastgheyb R, Barbee KA, Gallo G. Quantifying focal disruptions in axonal microtubules. In 2015 41st Annual Northeast Biomedical Engineering Conference, NEBEC 2015. Institute of Electrical and Electronics Engineers Inc. 2015. 7117155. (2015 41st Annual Northeast Biomedical Engineering Conference, NEBEC 2015). https://doi.org/10.1109/NEBEC.2015.7117155
Dastgheyb, Raha ; Barbee, K. A. ; Gallo, G. / Quantifying focal disruptions in axonal microtubules. 2015 41st Annual Northeast Biomedical Engineering Conference, NEBEC 2015. Institute of Electrical and Electronics Engineers Inc., 2015. (2015 41st Annual Northeast Biomedical Engineering Conference, NEBEC 2015).
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