Quantifying argonaute proteins in and out of GW/P-bodies: Implications in microRNA activities

Anthony K.L. Leung, Phillip A. Sharp

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

MicroRNAs (miRNAs) are a class of ∼22nt non-coding RNAs that regulate the translational potential and stability of mRNAs. Though constituting only 1-4% of human genes, miRNAs are predicted to regulate more than 60% of all mRNAs. The action of miRNAs is mediated through their associations with Argonaute proteins and mRNA targets. Previous studies indicated that though the majority of Argonaute proteins is diffusely distributed in the cytoplasm, a small fraction is consistently observed to be concentrated in a cytoplasmic compartment called GW/P-bodies. In this chapter, we will provide a quantitative and dynamic view of the subcellular localization of miRNA function, followed by a discussion on the possible roles of PBs in miRNA silencing.

Original languageEnglish (US)
Title of host publicationTen Years of Progress in GW/P Body Research
PublisherSpringer Science and Business Media, LLC
Pages165-182
Number of pages18
ISBN (Print)9781461451068
DOIs
StatePublished - 2013

Publication series

NameAdvances in Experimental Medicine and Biology
Volume768
ISSN (Print)0065-2598

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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