Quantification of the effects of mutations on receptor tyrosine kinase (RTK) activation in mammalian cells

Lijuan He; Kalina Hristova

doi:10.1007/978-1-4939-1789-1_8

Quantification of the effects of mutations on receptor tyrosine kinase (RTK) activation in mammalian cells

Lijuan He, Kalina Hristova

Whiting School of Engineering

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Single amino acid mutations in receptor tyrosine kinases (RTKs) are known to cause receptor overactivation and disease. Here we present a detailed protocol for the quantification of the effect of mutations on RTK activation in mammalian cells. The activation measurements are based on Western blotting, and involve direct comparison of receptor phosphorylation under conditions that ensure identical expression of wild-type and mutant receptors.

Original language	English (US)
Title of host publication	Receptor Tyrosine Kinases
Subtitle of host publication	Methods and Protocols
Publisher	Springer New York
Pages	81-87
Number of pages	7
ISBN (Electronic)	9781493917891
ISBN (Print)	9781493917884
DOIs	https://doi.org/10.1007/978-1-4939-1789-1_8
State	Published - Oct 15 2014

Keywords

Activation
Growth disorders
Phosphorylation
Receptor tyrosine kinase
Single amino acid mutation
Western blot

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Medicine(all)

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10.1007/978-1-4939-1789-1_8

Cite this

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abstract = "Single amino acid mutations in receptor tyrosine kinases (RTKs) are known to cause receptor overactivation and disease. Here we present a detailed protocol for the quantification of the effect of mutations on RTK activation in mammalian cells. The activation measurements are based on Western blotting, and involve direct comparison of receptor phosphorylation under conditions that ensure identical expression of wild-type and mutant receptors.",

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AU - Hristova, Kalina

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N2 - Single amino acid mutations in receptor tyrosine kinases (RTKs) are known to cause receptor overactivation and disease. Here we present a detailed protocol for the quantification of the effect of mutations on RTK activation in mammalian cells. The activation measurements are based on Western blotting, and involve direct comparison of receptor phosphorylation under conditions that ensure identical expression of wild-type and mutant receptors.

AB - Single amino acid mutations in receptor tyrosine kinases (RTKs) are known to cause receptor overactivation and disease. Here we present a detailed protocol for the quantification of the effect of mutations on RTK activation in mammalian cells. The activation measurements are based on Western blotting, and involve direct comparison of receptor phosphorylation under conditions that ensure identical expression of wild-type and mutant receptors.

KW - Activation

KW - Growth disorders

KW - Phosphorylation

KW - Receptor tyrosine kinase

KW - Single amino acid mutation

KW - Western blot

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BT - Receptor Tyrosine Kinases

PB - Springer New York

ER -

Quantification of the effects of mutations on receptor tyrosine kinase (RTK) activation in mammalian cells

Abstract

Keywords

ASJC Scopus subject areas

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