Abstract
Single amino acid mutations in receptor tyrosine kinases (RTKs) are known to cause receptor overactivation and disease. Here we present a detailed protocol for the quantification of the effect of mutations on RTK activation in mammalian cells. The activation measurements are based on Western blotting, and involve direct comparison of receptor phosphorylation under conditions that ensure identical expression of wild-type and mutant receptors.
Original language | English (US) |
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Title of host publication | Receptor Tyrosine Kinases |
Subtitle of host publication | Methods and Protocols |
Publisher | Springer New York |
Pages | 81-87 |
Number of pages | 7 |
ISBN (Electronic) | 9781493917891 |
ISBN (Print) | 9781493917884 |
DOIs | |
State | Published - Oct 15 2014 |
Keywords
- Activation
- Growth disorders
- Phosphorylation
- Receptor tyrosine kinase
- Single amino acid mutation
- Western blot
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Medicine(all)