Quantification of the effects of mutations on receptor tyrosine kinase (RTK) activation in mammalian cells

Lijuan He, Kalina Hristova

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Single amino acid mutations in receptor tyrosine kinases (RTKs) are known to cause receptor overactivation and disease. Here we present a detailed protocol for the quantification of the effect of mutations on RTK activation in mammalian cells. The activation measurements are based on Western blotting, and involve direct comparison of receptor phosphorylation under conditions that ensure identical expression of wild-type and mutant receptors.

Original languageEnglish (US)
Title of host publicationReceptor Tyrosine Kinases
Subtitle of host publicationMethods and Protocols
PublisherSpringer New York
Pages81-87
Number of pages7
ISBN (Electronic)9781493917891
ISBN (Print)9781493917884
DOIs
StatePublished - Oct 15 2014

Keywords

  • Activation
  • Growth disorders
  • Phosphorylation
  • Receptor tyrosine kinase
  • Single amino acid mutation
  • Western blot

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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