Quantification of paclitaxel metabolites in human plasma by high-performance liquid chromatography

M. T. Huizing, A. Sparreboom, H. Rosing, O. van Tellingen, H. M. Pinedo, J. H. Beijnen

Research output: Contribution to journalArticlepeer-review

Abstract

A reversed-phase high-performance liquid chromatographic (HPLC) method has been validated for the quantitative determination of the three major paclitaxel metabolites (6α-hydroxypaclitaxel, 3′-p-hydroxypaclitaxel, 6α,3′-p-dihydroxypaclitaxel) in human plasma. The HPLC system consists of an APEX-octyl analytical column and acetonitrile-methanol-0.02 M ammonium acetate buffer pH 5 (AMW; 4:1:5, v/v/v) as the mobile phase. Detection is performed by UV absorbance measurement at 227 nm. The sample pretreatment of the plasma samples involves solid-phase extraction (SPE) on Cyano Bond Elut columns. The concentrations of the metabolic products could be determined by using the paclitaxel standard curve with a correction factor of 1.14 for 6α,3′-p-dihydroxypaxlitaxel. The recoveries of paclitaxel and the metabolites 6α,3′-p-dihydroxypaclitaxel, 3′-p-hydroxypaclitaxel and 6α-hydroxypaclitaxel in human plasma were 89, 78, 91 and 89%, respectively. The accuracy of the assay for the determination of paclitaxel and its metabolites varied between 95 and 97%, at a 50 ng/ml analyte concentration. The lower limit of quantitation was 10 ng/ml for both the parent drug and its metabolites.

Original languageEnglish (US)
Pages (from-to)261-268
Number of pages8
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume674
Issue number2
DOIs
StatePublished - Dec 15 1995
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)

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