Quantification of optic nerve axon loss associated with a relative afferent pupillary defect in the monkey

John B. Kerrison, Kelvin Buchanan, Michael L. Rosenberg, Robert Clark, Kurt Andreason, Daniel V. Alfaro, Hans E. Grossniklaus, Lisa A. Kerrigan-Baumrind, Danielle F. Kerrigan, Neil R Miller, Harry A Quigley

Research output: Contribution to journalArticle

Abstract

Objective: To quantify the amount of optic nerve axonal loss associated with the presence of a mild relative afferent pupillary defect (RAPD) in an experimental monkey model. Methods: The right macula of 5 rhesus monkeys (Macaca mulatta) was treated with concentrically enlarging diode laser burns until an RAPD was detected using a transilluminator light and measured with neutral density filters. Intervals between treatments were 3 to 7 days over a period of 2 months. Pupillary responses to light stimulation were recorded with a monocular infrared television pupillometer. Two months after detection of an RAPD, 5 treated and 4 control monkeys underwent euthanasia and enucleation. Histopathologic analysis and quantification of optic nerve axon counts using an image analysis system were performed. Results: No RAPD was observed despite an estimated ganglion cell loss of up to 26%. A 0.6 log unit RAPD was present in 5 monkeys when the laser scar incorporated the entire macula within the temporal vascular arcades. One eye had progressive vitreomacular traction with worsening of the RAPD to 1.8 log units without further laser treatment. Histopathologic evaluation disclosed complete loss of the normal retinal architecture within the macula. The average fiber loss for the 4 treated eyes with 0.6 log unit RAPDs compared with fellow eyes was 53.3% (95% confidence interval [CI], 45.0%-61.6%). The average difference in axon counts between untreated pairs of optic nerves was 12.8% (95% CI, 10.0%-15.6%). Optic nerve axon loss between pairs of experimental and control eyes was statistically significant (P

Original languageEnglish (US)
Pages (from-to)1333-1341
Number of pages9
JournalArchives of Ophthalmology
Volume119
Issue number9
StatePublished - 2001

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Pupil Disorders
Optic Nerve
Haplorhini
Axons
Macaca mulatta
Lasers
Confidence Intervals
Light
Semiconductor Lasers
Euthanasia
Television
Traction
Ganglia
Cicatrix
Blood Vessels
Theoretical Models
Therapeutics

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Kerrison, J. B., Buchanan, K., Rosenberg, M. L., Clark, R., Andreason, K., Alfaro, D. V., ... Quigley, H. A. (2001). Quantification of optic nerve axon loss associated with a relative afferent pupillary defect in the monkey. Archives of Ophthalmology, 119(9), 1333-1341.

Quantification of optic nerve axon loss associated with a relative afferent pupillary defect in the monkey. / Kerrison, John B.; Buchanan, Kelvin; Rosenberg, Michael L.; Clark, Robert; Andreason, Kurt; Alfaro, Daniel V.; Grossniklaus, Hans E.; Kerrigan-Baumrind, Lisa A.; Kerrigan, Danielle F.; Miller, Neil R; Quigley, Harry A.

In: Archives of Ophthalmology, Vol. 119, No. 9, 2001, p. 1333-1341.

Research output: Contribution to journalArticle

Kerrison, JB, Buchanan, K, Rosenberg, ML, Clark, R, Andreason, K, Alfaro, DV, Grossniklaus, HE, Kerrigan-Baumrind, LA, Kerrigan, DF, Miller, NR & Quigley, HA 2001, 'Quantification of optic nerve axon loss associated with a relative afferent pupillary defect in the monkey', Archives of Ophthalmology, vol. 119, no. 9, pp. 1333-1341.
Kerrison JB, Buchanan K, Rosenberg ML, Clark R, Andreason K, Alfaro DV et al. Quantification of optic nerve axon loss associated with a relative afferent pupillary defect in the monkey. Archives of Ophthalmology. 2001;119(9):1333-1341.
Kerrison, John B. ; Buchanan, Kelvin ; Rosenberg, Michael L. ; Clark, Robert ; Andreason, Kurt ; Alfaro, Daniel V. ; Grossniklaus, Hans E. ; Kerrigan-Baumrind, Lisa A. ; Kerrigan, Danielle F. ; Miller, Neil R ; Quigley, Harry A. / Quantification of optic nerve axon loss associated with a relative afferent pupillary defect in the monkey. In: Archives of Ophthalmology. 2001 ; Vol. 119, No. 9. pp. 1333-1341.
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AU - Alfaro, Daniel V.

AU - Grossniklaus, Hans E.

AU - Kerrigan-Baumrind, Lisa A.

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