Quantification of nicotinic acetylcholine receptors in human brain using [123I]5-I-A-85380 SPET

Masahiro Fujita, Masanori Ichise, Christopher H. Van Dyck, Sami S. Zoghbi, Gilles Tamagnan, Alexey G. Mukhin, Ali Bozkurt, Nicholas Seneca, Dnyanesh Tipre, Christopher C. DeNucci, Hidehiro Iida, D. Bruce Vaupel, Andrew G. Horti, Andrei O. Koren, Alane S. Kimes, Edythe D. London, John P. Seibyl, Ronald M. Baldwin, Robert B. Innis

Research output: Contribution to journalArticle

Abstract

The purpose of this study was to assess the utility of a new single-photon emission tomography ligand, [ 123I]5-iodo-3-[2(S)-2-azetidinylmethoxy]pyridine (5-I-A-85380), to measure regional nAChR binding in human brain. Six healthy nonsmoker subjects (two men and four women, age 33±15 years) participated in both a bolus (dose: 317±42 MBq) and a bolus plus constant infusion (dose of bolus: 98±32 MBq, B/I=6.7±2.6 h, total dose: 331±55 MBq) study. The study duration was 5-8 h and 14 h in the former and the latter, respectively. Non-linear least-squares compartmental analysis was applied to bolus studies to calculate total (VT′) and specific (V S′) distribution volumes. A two-tissue compartment model was applied to identify VS′. VT′ was also calculated in B/I studies. In bolus studies, VT′ was well identified by both one- and two-tissue compartment models, with a coefficient of variation of less than 5% in most regions. The two-compartment model gave VT′ values of 51, 22, 27, 32, 20, 19, 20, and 17 ml cm -3 in thalamus, cerebellum, putamen, pons, and frontal, parietal, temporal, and occipital cortices, respectively. The two-compartment model did not identify VS′ well. B/I studies provided poor accuracy of VT′ measurement, possibly due to deviations from equilibrium conditions. These results demonstrate the feasibility of quantifying high-affinity type nAChRs using [123I]5-I-A-85380 in humans and support the use of VT′ measured by bolus studies.

Original languageEnglish (US)
Pages (from-to)1620-1629
Number of pages10
JournalEuropean Journal of Nuclear Medicine and Molecular Imaging
Volume30
Issue number12
DOIs
StatePublished - Dec 1 2003
Externally publishedYes

Keywords

  • Compartmental modeling
  • Distribution volume
  • Equilibrium
  • High-affinity type nicotinic receptors

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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    Fujita, M., Ichise, M., Van Dyck, C. H., Zoghbi, S. S., Tamagnan, G., Mukhin, A. G., Bozkurt, A., Seneca, N., Tipre, D., DeNucci, C. C., Iida, H., Vaupel, D. B., Horti, A. G., Koren, A. O., Kimes, A. S., London, E. D., Seibyl, J. P., Baldwin, R. M., & Innis, R. B. (2003). Quantification of nicotinic acetylcholine receptors in human brain using [123I]5-I-A-85380 SPET. European Journal of Nuclear Medicine and Molecular Imaging, 30(12), 1620-1629. https://doi.org/10.1007/s00259-003-1320-0