Quantification of neuroreceptors in the living human brain: III. D2- like dopamine receptors: Theory, validation, and changes during normal aging

Dean Foster Wong, David Young, P. David Wilson, Carolyn Cidis Meltzer, Albert Gjedde

Research output: Contribution to journalArticle

Abstract

Dopamine receptor density is believed to decline in normal aging. To test this hypothesis, we measured the density of dopamine D2-1ike receptors in vivo in the neostriatum of normal living humans by using the graphical method. This method determines the D2-like dopamine receptor density in the human brain with an occupying ligand (unlabeled haloperidol) and a radioligand (labeled 3-N-methylspiperone). The method was examined critically, and the assumptions underlying the method were shown to be valid. The validation included comparison of the representation of tracer metabolism by high-pressure liquid chromatography and model assays, calculation of the lumped constant D(w) from the value of its components, and comparable tracer partition coefficients in vitro and in vivo. In error analysis, the method consistently performed as well as the direct least-squares regression at statistical noise levels appropriate for the tomograph used in these studies. The method revealed that the density of the D2-like receptors that bind haloperidol in the caudate nucleus of normal humans declined 1% per year after the age of 18 years.

Original languageEnglish (US)
Pages (from-to)316-330
Number of pages15
JournalJournal of Cerebral Blood Flow and Metabolism
Volume17
Issue number3
StatePublished - Mar 1997

Fingerprint

Dopamine D2 Receptors
Sensory Receptor Cells
Brain
Haloperidol
Neostriatum
Caudate Nucleus
Dopamine Receptors
Least-Squares Analysis
High Pressure Liquid Chromatography
Regression Analysis
Ligands

Keywords

  • Dopamine receptors
  • Positron emission tomography
  • Receptor quantification
  • Schizophrenia

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

Cite this

Quantification of neuroreceptors in the living human brain : III. D2- like dopamine receptors: Theory, validation, and changes during normal aging. / Wong, Dean Foster; Young, David; Wilson, P. David; Meltzer, Carolyn Cidis; Gjedde, Albert.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 17, No. 3, 03.1997, p. 316-330.

Research output: Contribution to journalArticle

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