Quantification of latent tissue reservoirs and total body viral load in HIV-1 infection

Tae Wook Chun, Lucy Carruth, Diana Finzi, Xuefei Shen, Joseph A. DiGiuseppe, Harry Taylor, Monika Hermankova, Karen Chadwick, Joseph Bernard Margolick, Thomas C Quinn, Yen Hong Kuo, Ronald Brookmeyer, Martha A. Zeiger, Patricia Barditch-Crovo, Robert F Siliciano

Research output: Contribution to journalArticle

Abstract

The capacity of HIV-1 to establish latent infection of CD4+ T cells may allow viral persistence despite immune responses and anti-retroviral therapy. Measurements of infectious virus, and viral RNA in plasma and of infectious virus, viral DNA and viral messenger RNA species in infected cells all suggest that HIV-1 replication continues throughout the course of infection. Uncertainty remains over what fraction of CD4+ T cells are infected and whether there are latent reservoirs for the virus. We show here that during the asymptomatic phase of infection there is an extremely low total body load of latently infected resting CD4+ T cells with replication-competent integrated provirus (7 cells). The most prevalent form of HIV-1 DNA in resting and activated CD4+ T cells is a full-length, linear, unintegrated form that is not replication competent. The infection progresses even though at any given time in the lymphoid tissues integrated HIV-1 DNA is present in only a minute fraction of the susceptible populations, including resting and activated CD4+ T cells and macrophages.

Original languageEnglish (US)
Pages (from-to)183-188
Number of pages6
JournalNature
Volume387
Issue number6629
DOIs
StatePublished - May 8 1997

Fingerprint

Viral Load
HIV Infections
HIV-1
T-Lymphocytes
Viral RNA
Viruses
Infection
Proviruses
Asymptomatic Infections
DNA
Viral DNA
Lymphoid Tissue
Uncertainty
Macrophages
Messenger RNA
Population
Therapeutics

ASJC Scopus subject areas

  • General

Cite this

Chun, T. W., Carruth, L., Finzi, D., Shen, X., DiGiuseppe, J. A., Taylor, H., ... Siliciano, R. F. (1997). Quantification of latent tissue reservoirs and total body viral load in HIV-1 infection. Nature, 387(6629), 183-188. https://doi.org/10.1038/387183a0

Quantification of latent tissue reservoirs and total body viral load in HIV-1 infection. / Chun, Tae Wook; Carruth, Lucy; Finzi, Diana; Shen, Xuefei; DiGiuseppe, Joseph A.; Taylor, Harry; Hermankova, Monika; Chadwick, Karen; Margolick, Joseph Bernard; Quinn, Thomas C; Kuo, Yen Hong; Brookmeyer, Ronald; Zeiger, Martha A.; Barditch-Crovo, Patricia; Siliciano, Robert F.

In: Nature, Vol. 387, No. 6629, 08.05.1997, p. 183-188.

Research output: Contribution to journalArticle

Chun, TW, Carruth, L, Finzi, D, Shen, X, DiGiuseppe, JA, Taylor, H, Hermankova, M, Chadwick, K, Margolick, JB, Quinn, TC, Kuo, YH, Brookmeyer, R, Zeiger, MA, Barditch-Crovo, P & Siliciano, RF 1997, 'Quantification of latent tissue reservoirs and total body viral load in HIV-1 infection', Nature, vol. 387, no. 6629, pp. 183-188. https://doi.org/10.1038/387183a0
Chun TW, Carruth L, Finzi D, Shen X, DiGiuseppe JA, Taylor H et al. Quantification of latent tissue reservoirs and total body viral load in HIV-1 infection. Nature. 1997 May 8;387(6629):183-188. https://doi.org/10.1038/387183a0
Chun, Tae Wook ; Carruth, Lucy ; Finzi, Diana ; Shen, Xuefei ; DiGiuseppe, Joseph A. ; Taylor, Harry ; Hermankova, Monika ; Chadwick, Karen ; Margolick, Joseph Bernard ; Quinn, Thomas C ; Kuo, Yen Hong ; Brookmeyer, Ronald ; Zeiger, Martha A. ; Barditch-Crovo, Patricia ; Siliciano, Robert F. / Quantification of latent tissue reservoirs and total body viral load in HIV-1 infection. In: Nature. 1997 ; Vol. 387, No. 6629. pp. 183-188.
@article{89c010e802644dcb9fccfd2bc4d0eb2d,
title = "Quantification of latent tissue reservoirs and total body viral load in HIV-1 infection",
abstract = "The capacity of HIV-1 to establish latent infection of CD4+ T cells may allow viral persistence despite immune responses and anti-retroviral therapy. Measurements of infectious virus, and viral RNA in plasma and of infectious virus, viral DNA and viral messenger RNA species in infected cells all suggest that HIV-1 replication continues throughout the course of infection. Uncertainty remains over what fraction of CD4+ T cells are infected and whether there are latent reservoirs for the virus. We show here that during the asymptomatic phase of infection there is an extremely low total body load of latently infected resting CD4+ T cells with replication-competent integrated provirus (7 cells). The most prevalent form of HIV-1 DNA in resting and activated CD4+ T cells is a full-length, linear, unintegrated form that is not replication competent. The infection progresses even though at any given time in the lymphoid tissues integrated HIV-1 DNA is present in only a minute fraction of the susceptible populations, including resting and activated CD4+ T cells and macrophages.",
author = "Chun, {Tae Wook} and Lucy Carruth and Diana Finzi and Xuefei Shen and DiGiuseppe, {Joseph A.} and Harry Taylor and Monika Hermankova and Karen Chadwick and Margolick, {Joseph Bernard} and Quinn, {Thomas C} and Kuo, {Yen Hong} and Ronald Brookmeyer and Zeiger, {Martha A.} and Patricia Barditch-Crovo and Siliciano, {Robert F}",
year = "1997",
month = "5",
day = "8",
doi = "10.1038/387183a0",
language = "English (US)",
volume = "387",
pages = "183--188",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "6629",

}

TY - JOUR

T1 - Quantification of latent tissue reservoirs and total body viral load in HIV-1 infection

AU - Chun, Tae Wook

AU - Carruth, Lucy

AU - Finzi, Diana

AU - Shen, Xuefei

AU - DiGiuseppe, Joseph A.

AU - Taylor, Harry

AU - Hermankova, Monika

AU - Chadwick, Karen

AU - Margolick, Joseph Bernard

AU - Quinn, Thomas C

AU - Kuo, Yen Hong

AU - Brookmeyer, Ronald

AU - Zeiger, Martha A.

AU - Barditch-Crovo, Patricia

AU - Siliciano, Robert F

PY - 1997/5/8

Y1 - 1997/5/8

N2 - The capacity of HIV-1 to establish latent infection of CD4+ T cells may allow viral persistence despite immune responses and anti-retroviral therapy. Measurements of infectious virus, and viral RNA in plasma and of infectious virus, viral DNA and viral messenger RNA species in infected cells all suggest that HIV-1 replication continues throughout the course of infection. Uncertainty remains over what fraction of CD4+ T cells are infected and whether there are latent reservoirs for the virus. We show here that during the asymptomatic phase of infection there is an extremely low total body load of latently infected resting CD4+ T cells with replication-competent integrated provirus (7 cells). The most prevalent form of HIV-1 DNA in resting and activated CD4+ T cells is a full-length, linear, unintegrated form that is not replication competent. The infection progresses even though at any given time in the lymphoid tissues integrated HIV-1 DNA is present in only a minute fraction of the susceptible populations, including resting and activated CD4+ T cells and macrophages.

AB - The capacity of HIV-1 to establish latent infection of CD4+ T cells may allow viral persistence despite immune responses and anti-retroviral therapy. Measurements of infectious virus, and viral RNA in plasma and of infectious virus, viral DNA and viral messenger RNA species in infected cells all suggest that HIV-1 replication continues throughout the course of infection. Uncertainty remains over what fraction of CD4+ T cells are infected and whether there are latent reservoirs for the virus. We show here that during the asymptomatic phase of infection there is an extremely low total body load of latently infected resting CD4+ T cells with replication-competent integrated provirus (7 cells). The most prevalent form of HIV-1 DNA in resting and activated CD4+ T cells is a full-length, linear, unintegrated form that is not replication competent. The infection progresses even though at any given time in the lymphoid tissues integrated HIV-1 DNA is present in only a minute fraction of the susceptible populations, including resting and activated CD4+ T cells and macrophages.

UR - http://www.scopus.com/inward/record.url?scp=0030913366&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030913366&partnerID=8YFLogxK

U2 - 10.1038/387183a0

DO - 10.1038/387183a0

M3 - Article

VL - 387

SP - 183

EP - 188

JO - Nature

JF - Nature

SN - 0028-0836

IS - 6629

ER -