@inbook{9e9ddec3ac504494a5d78395a83f6ac9,
title = "Quantification of Infectious SARS-CoV-2 by the 50% Tissue Culture Infectious Dose Endpoint Dilution Assay",
abstract = "A number of viral quantification methods are used to measure the concentration of infectious severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). While the traditional plaque-based assay allows for direct enumeration of replication competent lytic virions and remains the gold standard for the quantification of infectious virus, the 50% tissue culture infectious dose (TCID50) endpoint dilution assay allows for a more rapid, large-scale analysis of experimental samples. In this chapter, we describe a well-established TCID50 assay protocol to measure the SARS-CoV-2 infectious titer in viral stocks, in vitro cell or organoid models, and animal tissue. We also present alternative assays for scoring the cytopathic effect of SARS-CoV-2 in cell culture and comparable methods to calculate the 50% endpoint by serial dilution.",
keywords = "Cell culture, Cell viability assay, Cytopathic effects (CPE), Karber, Reed and Muench, SARS-CoV-2, TCID, Trimmed Spearman, Virus quantification",
author = "Bullen, {C. Korin} and Davis, {Stephanie L.} and Looney, {Monika M.}",
note = "Funding Information: The authors would like to thank Dr. Andrew S. Pekosz and the members of his laboratory at The Johns Hopkins School of Public Health for guidance, materials, and training on quantification of SARS-CoV-2 and for providing their TCID50 protocol from which we further developed the methods described herein. We are grateful to Kirsten Littlefield who took the time out of her busy schedule during an emerging SARS pandemic to educate us on CPE morphology in Vero-E6 cells and TCID50 calculation methods. We would also like to thank Dr. William R. Bishai for his valuable scientific discussions and logistical support. The Mercatus Center Fast Grant Award #2167 funded this work. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2111-0_9",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "131--146",
booktitle = "Methods in Molecular Biology",
}