Quantification of hydroxychloroquine in blood using turbulent flow liquid chromatography-tandem mass spectrometry (TFLC-MS/MS)

Allison B. Chambliss, Anna K. Füzéry, William A. Clarke

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Hydroxychloroquine (HQ) is used routinely in the treatment of autoimmune disorders such as rheumatoid arthritis and lupus erythematosus. Issues such as marked pharmacokinetic variability and patient noncompliance make therapeutic drug monitoring of HQ a useful tool for management of patients taking this drug. Quantitative measurements of HQ may aid in identifying poor efficacy as well as provide reliable information to distinguish patient non-compliance from refractory disease. We describe a rapid 7-min assay for the accurate and precise measurement of HQ concentrations in 100 μL samples of human blood using turbulent flow liquid chromatography coupled to tandem mass spectrometry. HQ is isolated from EDTA whole blood after a simple extraction with its deuterated analog, hydroxychloroquine-d4, in 0.33 M per-chloric acid. Samples are then centrifuged and injected onto the TFLC-MS/MS system. Quantification is performed using a nine-point calibration curve that is linear over a wide range (15.7-4000 ng/mL) with precisions of <5%.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages177-184
Number of pages8
Volume1383
DOIs
Publication statusPublished - May 1 2016

Publication series

NameMethods in Molecular Biology
Volume1383
ISSN (Print)10643745

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Keywords

  • Blood
  • Hydroxychloroquine
  • Quantification
  • Tandem mass spectrometry
  • Therapeutic drug monitoring
  • Turbulent flow liquid chromatography

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Chambliss, A. B., Füzéry, A. K., & Clarke, W. A. (2016). Quantification of hydroxychloroquine in blood using turbulent flow liquid chromatography-tandem mass spectrometry (TFLC-MS/MS). In Methods in Molecular Biology (Vol. 1383, pp. 177-184). (Methods in Molecular Biology; Vol. 1383). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3252-8_19