Quantification of docetaxel in serum using turbulent flow liquid chromatography electrospray tandem mass spectrometry (TFC-HPLC-ESI-MS/MS)

Christopher A. Crutchfield, Mark A. Marzinke, William A. Clarke

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Docetaxel is a second-generation taxane and is used clinically as an anti-neoplastic agent in cancer chemotherapy via an anti-mitotic mechanism. Its efficacy is limited to a narrow therapeutic window. Inappropriately high concentrations may cause erythema, fluid retention, nausea, diarrhea, and neutropenia. As a result, dosing recommendations have changed from high dosage loading every 3 weeks to lower dosage loading weekly. We describe a method that can be used for therapeutic drug monitoring of docetaxel levels using turbulent flow liquid chromatography electrospray tandem mass spectrometry (TFC-HPLC-ESI-MS/ MS). The method is rapid, requiring only 6.3 min per analytical run following a simple protein crash. The method requires only 100 μL of serum. Concentrations of docetaxel were quantified by a calibration curve relating the peak-area ratio of docetaxel to a deuterated internal standard (docetaxel-D9). The method was linear from 7.8 to 1000 ng/mL, with imprecision ≤6.2%.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages121-124
Number of pages4
Volume1383
DOIs
Publication statusPublished - May 1 2016

Publication series

NameMethods in Molecular Biology
Volume1383
ISSN (Print)10643745

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Keywords

  • Anti-neoplastic
  • Docetaxel
  • Turbulent flow liquid chromatography electrospray tandem mass spectrometry

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Crutchfield, C. A., Marzinke, M. A., & Clarke, W. A. (2016). Quantification of docetaxel in serum using turbulent flow liquid chromatography electrospray tandem mass spectrometry (TFC-HPLC-ESI-MS/MS). In Methods in Molecular Biology (Vol. 1383, pp. 121-124). (Methods in Molecular Biology; Vol. 1383). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3252-8_14