Quake: Quality-aware detection and correction of sequencing errors

David R. Kelley, Michael C. Schatz, Steven L Salzberg

Research output: Contribution to journalArticle


We introduce Quake, a program to detect and correct errors in DNA sequencing reads. Using a maximum likelihood approach incorporating quality values and nucleotide specific miscall rates, Quake achieves the highest accuracy on realistically simulated reads. We further demonstrate substantial improvements in de novo assembly and SNP detection after using Quake. Quake can be used for any size project, including more than one billion human reads, and is freely available as open source software from http://www.cbcb.umd.edu/software/quake.

Original languageEnglish (US)
Article numberR116
JournalGenome Biology
Issue number11
StatePublished - Nov 29 2010
Externally publishedYes


ASJC Scopus subject areas

  • Genetics
  • Cell Biology
  • Ecology, Evolution, Behavior and Systematics

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