Qa-region class I gene expression

Identification of a second class I gene, Q9, encoding a Qa-2 polypeptide

Mark J Soloski, L. Hood, I. Stroynowski

Research output: Contribution to journalArticle

Abstract

A feature of the expression of the tissue-specific class I antigen Qa-2 is the quantitative variation among mouse strains. Recently, the class I gene Q7 has been shown to encode a protein product that is biochemically indistinguishable from the lymphocyte-bound Qa-2 molecule. Utilizing gene transfection, we have identified a second Qa-2 subregion class I gene (Q9), in H-2b mice, which encodes a polypeptide biochemically similar to the Q7 and the Qa-2 polypeptides. Furthermore, we have observed that cell lines transfected with the allelic forms of the Q7 gene from C57BL/10 (Qa-2(hi)) or BALB/C (Qa-2(low)) display quantitative differences in cell-surface expression. Based on these studies, we suggest that gene dosage and allele-specific variation in cell-surface expression contribute to the strain-specific variation in the levels of Qa-2 antigen expression.

Original languageEnglish (US)
Pages (from-to)3100-3104
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number9
StatePublished - 1988

Fingerprint

MHC Class I Genes
Gene Expression
Histocompatibility Antigens Class I
Peptides
Gene Dosage
Genes
Transfection
Alleles
Lymphocytes
Cell Line
Proteins
Q surface antigens

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

@article{0c00f1d71b354fa3b3883b108286359b,
title = "Qa-region class I gene expression: Identification of a second class I gene, Q9, encoding a Qa-2 polypeptide",
abstract = "A feature of the expression of the tissue-specific class I antigen Qa-2 is the quantitative variation among mouse strains. Recently, the class I gene Q7 has been shown to encode a protein product that is biochemically indistinguishable from the lymphocyte-bound Qa-2 molecule. Utilizing gene transfection, we have identified a second Qa-2 subregion class I gene (Q9), in H-2b mice, which encodes a polypeptide biochemically similar to the Q7 and the Qa-2 polypeptides. Furthermore, we have observed that cell lines transfected with the allelic forms of the Q7 gene from C57BL/10 (Qa-2(hi)) or BALB/C (Qa-2(low)) display quantitative differences in cell-surface expression. Based on these studies, we suggest that gene dosage and allele-specific variation in cell-surface expression contribute to the strain-specific variation in the levels of Qa-2 antigen expression.",
author = "Soloski, {Mark J} and L. Hood and I. Stroynowski",
year = "1988",
language = "English (US)",
volume = "85",
pages = "3100--3104",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "9",

}

TY - JOUR

T1 - Qa-region class I gene expression

T2 - Identification of a second class I gene, Q9, encoding a Qa-2 polypeptide

AU - Soloski, Mark J

AU - Hood, L.

AU - Stroynowski, I.

PY - 1988

Y1 - 1988

N2 - A feature of the expression of the tissue-specific class I antigen Qa-2 is the quantitative variation among mouse strains. Recently, the class I gene Q7 has been shown to encode a protein product that is biochemically indistinguishable from the lymphocyte-bound Qa-2 molecule. Utilizing gene transfection, we have identified a second Qa-2 subregion class I gene (Q9), in H-2b mice, which encodes a polypeptide biochemically similar to the Q7 and the Qa-2 polypeptides. Furthermore, we have observed that cell lines transfected with the allelic forms of the Q7 gene from C57BL/10 (Qa-2(hi)) or BALB/C (Qa-2(low)) display quantitative differences in cell-surface expression. Based on these studies, we suggest that gene dosage and allele-specific variation in cell-surface expression contribute to the strain-specific variation in the levels of Qa-2 antigen expression.

AB - A feature of the expression of the tissue-specific class I antigen Qa-2 is the quantitative variation among mouse strains. Recently, the class I gene Q7 has been shown to encode a protein product that is biochemically indistinguishable from the lymphocyte-bound Qa-2 molecule. Utilizing gene transfection, we have identified a second Qa-2 subregion class I gene (Q9), in H-2b mice, which encodes a polypeptide biochemically similar to the Q7 and the Qa-2 polypeptides. Furthermore, we have observed that cell lines transfected with the allelic forms of the Q7 gene from C57BL/10 (Qa-2(hi)) or BALB/C (Qa-2(low)) display quantitative differences in cell-surface expression. Based on these studies, we suggest that gene dosage and allele-specific variation in cell-surface expression contribute to the strain-specific variation in the levels of Qa-2 antigen expression.

UR - http://www.scopus.com/inward/record.url?scp=0011787432&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0011787432&partnerID=8YFLogxK

M3 - Article

VL - 85

SP - 3100

EP - 3104

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 9

ER -