Qa-2, H-2K, and H-2D alloantigens evolved from a common ancestral gene

Mark J Soloski, J. W. Uhr, L. Flaherty, E. S. Vitetta

Research output: Contribution to journalArticle

Abstract

The Tla region located on the murine 17th chromosome controls several serologically defined cell surface antigens. These antigens, referred to as Qa-1-5 and TL, are expressed on a variety of hematopoeitic cell populations. In the present studies we have immunoprecipitated isotopically labeled Qa-2 and H-2 molecules from mitogen-stimulated B6 spleen cells. Sequential immunoprecipitation experiments have shown that the determinants recognized by αQa-2, αH-2K6, and αH-2D6 alloantisera reside on separate molecular species. Comparative mapping of the arginine-labeled tryptic peptides from Qa-2, H-2K6, and H-2D6 molecules indicate that Qa-2 is structurally distinct but that there is considerable structual homology; 21-43% of the Qa-2 peptides co-chromatograph with peptides derived from H-2Db and H-2Kb, respectively. Similar levels of homology are observed when Qa-2 is compared with H-2K(k) or H-2D(d). The results show that the Qa-2 alloantigen is encoded by a locus separate from the loci encoding H-2K or H-2D alloantigens, but that the Qa-2, H-2K, and H-2D alloantigens are sufficiently related at the primary structural level to indicate that they evolved from a common primordial gene.

Original languageEnglish (US)
Pages (from-to)1080-1093
Number of pages14
JournalJournal of Experimental Medicine
Volume153
Issue number5
StatePublished - 1981
Externally publishedYes

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Isoantigens
Peptides
Genes
Surface Antigens
Mitogens
Immunoprecipitation
Arginine
Spleen
Chromosomes
Antigens
Population

ASJC Scopus subject areas

  • Immunology

Cite this

Qa-2, H-2K, and H-2D alloantigens evolved from a common ancestral gene. / Soloski, Mark J; Uhr, J. W.; Flaherty, L.; Vitetta, E. S.

In: Journal of Experimental Medicine, Vol. 153, No. 5, 1981, p. 1080-1093.

Research output: Contribution to journalArticle

Soloski, MJ, Uhr, JW, Flaherty, L & Vitetta, ES 1981, 'Qa-2, H-2K, and H-2D alloantigens evolved from a common ancestral gene', Journal of Experimental Medicine, vol. 153, no. 5, pp. 1080-1093.
Soloski, Mark J ; Uhr, J. W. ; Flaherty, L. ; Vitetta, E. S. / Qa-2, H-2K, and H-2D alloantigens evolved from a common ancestral gene. In: Journal of Experimental Medicine. 1981 ; Vol. 153, No. 5. pp. 1080-1093.
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