Pyrosequencing to resolve discrepant Xpert MTB/RIF and mycobacterial growth indicator tube 960

Kanchan Ajbani, Mubin Kazi, Jeffrey Tornheim, Swapna Naik, Rajeev Soman, Anjali Shetty, Camilla Rodrigues

Research output: Contribution to journalArticle

Abstract

Delayed diagnosis of drug resistance has been a major obstacle to proper management and control of drug-resistant tuberculosis (TB). Expanded access to rapid molecular diagnostics such as Xpert MTB/RIF has been helpful, but has generated confusion about how to interpret genotype-phenotype discordance. Optimal management is not clearly defined for patients with rifampin resistance by Xpert MTB/RIF but rifampin susceptibility by phenotypic testing. To resolve this discrepancy, we performed pyrosequencing of discordant isolates identified at a reference laboratory over a 6-month period. We present here strategies to address genotype-phenotype discordance using sequencing.

Original languageEnglish (US)
Pages (from-to)168-170
Number of pages3
JournalLung India
Volume35
Issue number2
DOIs
StatePublished - Mar 1 2018

Keywords

  • Culture
  • Discrepancy
  • Pyrosequencing
  • Xpert

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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