Pyrimidine motif triplexes containing polypurine RNA or DNA with oligo 2'-O-methyl or DNA triplex forming oligonucleotides

Marc Beban, Paul S. Miller

Research output: Contribution to journalArticle

Abstract

Triplex forming oligonucleotides (TFOs) are potentially useful in targeting RNA for antisense therapeutic applications. To determine the feasibility of targeting polypurine RNA with nuclease-resistant oligonucleotides, TFOs containing 2'-deoxy or 2'-O-methyl (2'-OMe) backbones, designed to form pyrimidine motif triplexes with RNA, were synthesized. TFOs were made which can form trimolecular triplexes, or bimolecular, 'clamp' triplexes with polypurine RNA and DNA. It was found that the relative stabilities of the triplexes formed followed the order: M·DM(clamp)>>>D·DD˜M·DD>M·RM>D·DM>M·RD˜M·DM, where M is a 2'-OMe, D is a DNA and R is an RNA backbone. The third strand is listed first, separated by a dot from the purine strand of the Watson-Crick duplex, followed by the pyrimidine strand of the duplex. The results described here provide insight into the feasibility of using TFOs containing a 2'-OMe backbone as antisense agents.

Original languageEnglish (US)
Pages (from-to)155-162
Number of pages8
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1492
Issue number1
DOIs
StatePublished - Jun 21 2000

Keywords

  • 2'-O-Methyl
  • Antisense
  • RNA
  • Triplex forming oligonucleotide

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

Fingerprint Dive into the research topics of 'Pyrimidine motif triplexes containing polypurine RNA or DNA with oligo 2'-O-methyl or DNA triplex forming oligonucleotides'. Together they form a unique fingerprint.

  • Cite this