Pylorectomy reduces the satiety action of cholecystokinin

Timothy H Moran, L. Shnayder, A. M. Hostetler, Paul R McHugh

Research output: Contribution to journalArticle

Abstract

Rat gastric cholecystokinin (CCK) receptors are localized to the circular muscle layer of the pyloric sphincter, and a role for these receptors in the mediation of CCK satiety has been proposed. To directly assess the contribution of this receptor population in CCK satiety, the area of the pyloric sphincter containing these receptors was surgically removed, and the behavioral responses to CCK were compared pre- and postpylorectomy. The presence of CCK receptors in the gastroduodenal junction was assessed by either in vitro CCK recetor autoradiography or in vitro contractile response to CCK. The results depended on the time after pylorectomy during which testing occurred. Two to 3 wk after pylorectomy rats demonstrated a significant attenuation of CCK satiety such that while the response to 1 and 2 μg/kg was intact, any additional inhibition by 4 and 8 μg/kg was eliminated. At this time, no evidence of CCK receptors around the gastroduodenal junction was found. In contrast, 2-3 mo after pylorectomy, the normal dose-response inhibition to CCK was intact. Evidence for the presence of CCK binding sites at the gastroduodenal junction was found by both autoradiography and physiological assessment. These results indicate a role for pyloric CCK receptors in the mediation of CCK satiety.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume255
Issue number6
StatePublished - 1988

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Cholecystokinin
Cholecystokinin Receptors
Pylorus
Autoradiography
Stomach
Binding Sites
Muscles

ASJC Scopus subject areas

  • Physiology

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Pylorectomy reduces the satiety action of cholecystokinin. / Moran, Timothy H; Shnayder, L.; Hostetler, A. M.; McHugh, Paul R.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 255, No. 6, 1988.

Research output: Contribution to journalArticle

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