Putative mannose-specific phosphotransferase system component IID represses expression of suilysin in serotype 2 Streptococcus suis

Shichun Lun, P. J. Willson

Research output: Contribution to journalArticle

Abstract

In this study, we generated a genomic mutant library from a North American strain of serotype 2 Streptococcus suis using the pGh9:ISS1 transposition vector. Suilysin is the hemolysin made by S. suis. A hyper-hemolytic mutant was identified by screening for hemolytic phenotype using media with human blood. The hyper-hemolytic phenotype was characterised by a quantitative hemolysis microplate method. The use of green fluorescent protein (GFP) as a reporter also showed that suilysin gene expression was greater in the mutant. DNA sequence analysis of 3.8 kb surrounding the ISS1 insertion site revealed four open reading frames (ORFs) with three consecutive ORFs that belong to a putative mannose-specific phosphotransferase system (PTS). The S. suis gene homologous to mannose permease IID, manN, was interrupted by the transposon. A complementation test showed that manN repressed the expression of suilysin and the absence of manN was responsible for the hyper-hemolytic phenotype. However, both wild type and isogenic hyper-hemolytic mutant S. suis fermented mannose, glucose and lactose. Thus, despite its potential roles in carbohydrate transport, phosphorylation and metabolism, the manN homologue in the putative mannose-specific PTS regulates gene expression in S. suis.

Original languageEnglish (US)
Pages (from-to)169-180
Number of pages12
JournalVeterinary Microbiology
Volume105
Issue number3-4
DOIs
StatePublished - Feb 25 2005

Fingerprint

Streptococcus suis
Mannose
mannose
serotypes
phosphotransferases (kinases)
Phosphotransferases
mutants
Phenotype
phenotype
Open Reading Frames
open reading frames
Genetic Complementation Test
Gene Expression
gene expression
Hemolysin Proteins
hemolysins
Genomic Library
transposition (genetics)
hemolysis
Lactose

Keywords

  • Gene regulation
  • Mannose-specific phosphotransferase system
  • Mutagenesis
  • Streptococcus suis
  • Suilysin

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

Cite this

Putative mannose-specific phosphotransferase system component IID represses expression of suilysin in serotype 2 Streptococcus suis. / Lun, Shichun; Willson, P. J.

In: Veterinary Microbiology, Vol. 105, No. 3-4, 25.02.2005, p. 169-180.

Research output: Contribution to journalArticle

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