Pushing the Bacterial Envelope: Strategies for Re-Engineering Bacterial Surfaces with Heterologous Proteins and Sugars

Samir Gautam, David A. Spiegel

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Over the past three decades, a powerful array of techniques has been developed for expressing heterologous proteins and saccharides on the surface of bacteria. Surface-engineered bacteria, in turn, have proven useful in a variety of settings, including high-throughput screening, biofuel production, and vaccinology. In this chapter, we provide a comprehensive review of methods for displaying polypeptides and sugars on the bacterial cell surface, and discuss the many innovative applications these methods have found to date. While already an important biotechnological tool, we believe bacterial surface display may be further improved through integration with emerging methodology in other fields, such as protein engineering and synthetic chemistry. Ultimately, we envision bacterial display becoming a multidisciplinary platform with the potential to transform basic and applied research in bacteriology, biotechnology, and biomedicine.

Original languageEnglish (US)
Title of host publicationMicro-and Nanoengineering of the Cell Surface
PublisherElsevier Inc.
Pages63-97
Number of pages35
ISBN (Electronic)9781455731558
ISBN (Print)9781455731466
DOIs
StatePublished - Jun 6 2014
Externally publishedYes

Fingerprint

Sugars
Proteins
Bacteriology
Bacteria
Display devices
Polypeptides
Biofuels
Biotechnology
Screening
Throughput

Keywords

  • Bacterial envelope
  • Biocatalysis
  • Cancer therapy
  • Glycoengineering
  • Heterologous expression systems
  • High-throughput screening
  • Probiotics
  • Surface engineering
  • Vaccine design

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Pushing the Bacterial Envelope : Strategies for Re-Engineering Bacterial Surfaces with Heterologous Proteins and Sugars. / Gautam, Samir; Spiegel, David A.

Micro-and Nanoengineering of the Cell Surface. Elsevier Inc., 2014. p. 63-97.

Research output: Chapter in Book/Report/Conference proceedingChapter

Gautam, Samir ; Spiegel, David A. / Pushing the Bacterial Envelope : Strategies for Re-Engineering Bacterial Surfaces with Heterologous Proteins and Sugars. Micro-and Nanoengineering of the Cell Surface. Elsevier Inc., 2014. pp. 63-97
@inbook{f4cbfa2ec2fd47c19b6146e1ba6f8c53,
title = "Pushing the Bacterial Envelope: Strategies for Re-Engineering Bacterial Surfaces with Heterologous Proteins and Sugars",
abstract = "Over the past three decades, a powerful array of techniques has been developed for expressing heterologous proteins and saccharides on the surface of bacteria. Surface-engineered bacteria, in turn, have proven useful in a variety of settings, including high-throughput screening, biofuel production, and vaccinology. In this chapter, we provide a comprehensive review of methods for displaying polypeptides and sugars on the bacterial cell surface, and discuss the many innovative applications these methods have found to date. While already an important biotechnological tool, we believe bacterial surface display may be further improved through integration with emerging methodology in other fields, such as protein engineering and synthetic chemistry. Ultimately, we envision bacterial display becoming a multidisciplinary platform with the potential to transform basic and applied research in bacteriology, biotechnology, and biomedicine.",
keywords = "Bacterial envelope, Biocatalysis, Cancer therapy, Glycoengineering, Heterologous expression systems, High-throughput screening, Probiotics, Surface engineering, Vaccine design",
author = "Samir Gautam and Spiegel, {David A.}",
year = "2014",
month = "6",
day = "6",
doi = "10.1016/B978-1-4557-3146-6.00004-0",
language = "English (US)",
isbn = "9781455731466",
pages = "63--97",
booktitle = "Micro-and Nanoengineering of the Cell Surface",
publisher = "Elsevier Inc.",

}

TY - CHAP

T1 - Pushing the Bacterial Envelope

T2 - Strategies for Re-Engineering Bacterial Surfaces with Heterologous Proteins and Sugars

AU - Gautam, Samir

AU - Spiegel, David A.

PY - 2014/6/6

Y1 - 2014/6/6

N2 - Over the past three decades, a powerful array of techniques has been developed for expressing heterologous proteins and saccharides on the surface of bacteria. Surface-engineered bacteria, in turn, have proven useful in a variety of settings, including high-throughput screening, biofuel production, and vaccinology. In this chapter, we provide a comprehensive review of methods for displaying polypeptides and sugars on the bacterial cell surface, and discuss the many innovative applications these methods have found to date. While already an important biotechnological tool, we believe bacterial surface display may be further improved through integration with emerging methodology in other fields, such as protein engineering and synthetic chemistry. Ultimately, we envision bacterial display becoming a multidisciplinary platform with the potential to transform basic and applied research in bacteriology, biotechnology, and biomedicine.

AB - Over the past three decades, a powerful array of techniques has been developed for expressing heterologous proteins and saccharides on the surface of bacteria. Surface-engineered bacteria, in turn, have proven useful in a variety of settings, including high-throughput screening, biofuel production, and vaccinology. In this chapter, we provide a comprehensive review of methods for displaying polypeptides and sugars on the bacterial cell surface, and discuss the many innovative applications these methods have found to date. While already an important biotechnological tool, we believe bacterial surface display may be further improved through integration with emerging methodology in other fields, such as protein engineering and synthetic chemistry. Ultimately, we envision bacterial display becoming a multidisciplinary platform with the potential to transform basic and applied research in bacteriology, biotechnology, and biomedicine.

KW - Bacterial envelope

KW - Biocatalysis

KW - Cancer therapy

KW - Glycoengineering

KW - Heterologous expression systems

KW - High-throughput screening

KW - Probiotics

KW - Surface engineering

KW - Vaccine design

UR - http://www.scopus.com/inward/record.url?scp=84941779793&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84941779793&partnerID=8YFLogxK

U2 - 10.1016/B978-1-4557-3146-6.00004-0

DO - 10.1016/B978-1-4557-3146-6.00004-0

M3 - Chapter

AN - SCOPUS:84941779793

SN - 9781455731466

SP - 63

EP - 97

BT - Micro-and Nanoengineering of the Cell Surface

PB - Elsevier Inc.

ER -