Puromycin resistance in Chinese hamster cells: Genetic and biochemical studies of partially resistant, unstable clones

John Morrow, David Sammons, Emily Barron

Research output: Contribution to journalArticlepeer-review

Abstract

Resistance to 10 μg/ml of puromycin has been analyzed in V79 Chinese hamster cells. Clones that were isolated in 10 μg/ml of puromycin and subsequently cultivated in its absence consistently lost their resistance. One clone was analyzed in detail by recloning in the presence and absence of puromycin, and it was found that non-puromycin cultivated sublones also lost their resistance and regained inhibition profiles similar to the V79 parent. Reconstruction experiments between sensitive and resistant cells demonstrated that the yield of mutants was not affected by metabolic cooperation. The mutation rate was calculated to be 1 × 10-7 per cell per generation, and was the same within the limits of statistical error in a colchicine-produced polyploid derivative of the V79 line. Although a number of resistant clones were found to have polyploid karyotypes, the polyploid V79 line was not more resistant to puromycin, nor did it possess a higher frequency of puromycin resistant cells. Studies employing radiolabeled puromycin established that resistance was due to a lowered uptake of puromycin and that an inverse relationship existed between resistance level and uptake rate.

Original languageEnglish (US)
Pages (from-to)333-346
Number of pages14
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume69
Issue number2
DOIs
StatePublished - Feb 1980
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis

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