TY - JOUR
T1 - Purified reconstituted inositol 1,4,5-trisphosphate receptors
T2 - Thiol reagents act directly on receptor protein
AU - Kaplin, Adam I.
AU - Ferris, Christopher D.
AU - Voglmaier, Susan M.
AU - Snyder, Solomon H.
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1994/11/18
Y1 - 1994/11/18
N2 - Thimerosal, a sulfhydryl oxidizing reagent, has been shown to induce Ca2+ mobilization in several cell types and to increase the sensitivity of intracellular Ca2+ stores to inositol 1,4,5-trisphosphate (IP3). Using purified IP3 receptor (IP3R) protein reconstituted in vesicles, we demonstrate pronounced stimulation by thimerosal of its Ca2+ channel activity. Effects of thimerosal are dependent on the redox state of the receptor, implying an action of thimerosal on a critical sulfhydryl group(s) of IP3R. Thimerosal enhances the affinity of IP3R for IP3 binding. The manner in which thimerosal modulates IP3R responsiveness to IP3 provides evidence for receptor heterogeneity with implications for mechanisms of quantal Ca2+ release. These results clarify regulation of IP3R activity by redox modulation.
AB - Thimerosal, a sulfhydryl oxidizing reagent, has been shown to induce Ca2+ mobilization in several cell types and to increase the sensitivity of intracellular Ca2+ stores to inositol 1,4,5-trisphosphate (IP3). Using purified IP3 receptor (IP3R) protein reconstituted in vesicles, we demonstrate pronounced stimulation by thimerosal of its Ca2+ channel activity. Effects of thimerosal are dependent on the redox state of the receptor, implying an action of thimerosal on a critical sulfhydryl group(s) of IP3R. Thimerosal enhances the affinity of IP3R for IP3 binding. The manner in which thimerosal modulates IP3R responsiveness to IP3 provides evidence for receptor heterogeneity with implications for mechanisms of quantal Ca2+ release. These results clarify regulation of IP3R activity by redox modulation.
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M3 - Article
C2 - 7961860
AN - SCOPUS:0028151010
SN - 0021-9258
VL - 269
SP - 28972
EP - 28978
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 46
ER -